CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015580
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TOM1-like protein 2 
Protein Synonyms/Alias
 Target of Myb-like protein 2 
Gene Name
 TOM1L2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
101FIDSVLVKIISPKNNubiquitination[1, 2]
106LVKIISPKNNPPTIVubiquitination[1, 2, 3, 4, 5, 6, 7]
145VHIYEELKRKGVEFPubiquitination[1, 2]
147IYEELKRKGVEFPMAubiquitination[1, 2, 7]
236DVVRGNTKVMSEMLTubiquitination[3]
400NSLAEQRKTVTYEDPubiquitination[1, 2, 3, 6, 7, 8]
421ASALDNRKQSSEGIPubiquitination[3, 9]
447VWLRTDLKGDDLEEGubiquitination[3]
462VTSEEFDKFLEERAKubiquitination[1, 2]
469KFLEERAKAAEMVPDubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [9] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
 Probable role in protein transport. May regulate growth factor-induced mitogenic signaling. 
Sequence Annotation
 DOMAIN 20 152 VHS.
 DOMAIN 219 307 GAT.
 MOTIF 329 334 Clathrin-binding.
 MOD_RES 160 160 Phosphoserine.
 MOD_RES 164 164 Phosphothreonine.
 MOD_RES 479 479 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 507 AA 
Protein Sequence
MEFLLGNPFS TPVGQCLEKA TDGSLQSEDW TLNMEICDII NETEEGPKDA IRALKKRLNG 60
NRNYREVMLA LTVLETCVKN CGHRFHILVA NRDFIDSVLV KIISPKNNPP TIVQDKVLAL 120
IQAWADAFRS SPDLTGVVHI YEELKRKGVE FPMADLDALS PIHTPQRSVP EVDPAATMPR 180
SQSQQRTSAG SYSSPPPAPY SAPQAPALSV TGPITANSEQ IARLRSELDV VRGNTKVMSE 240
MLTEMVPGQE DSSDLELLQE LNRTCRAMQQ RIVELISRVS NEEVTEELLH VNDDLNNVFL 300
RYERFERYRS GRSVQNASNG VLNEVTEDNL IDLGPGSPAV VSPMVGNTAP PSSLSSQLAG 360
LDLGTESVSG TLSSLQQCNP RDGFDMFAQT RGNSLAEQRK TVTYEDPQAV GGLASALDNR 420
KQSSEGIPVA QPSVMDDIEV WLRTDLKGDD LEEGVTSEEF DKFLEERAKA AEMVPDLPSP 480
PMEAPAPASN PSGRKKPERS EDALFAL 507 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro. 
Interpro
 IPR008942; ENTH_VHS.
 IPR004152; GAT.
 IPR014645; TOM1.
 IPR027429; TOM1L2.
 IPR002014; VHS.
 IPR018205; VHS_subgr. 
Pfam
 PF03127; GAT
 PF00790; VHS 
SMART
 SM00288; VHS 
PROSITE
 PS50909; GAT
 PS50179; VHS 
PRINTS