UniProt Accession

 MYL6_HUMAN; P60660; P16475; P24572; P24573; Q12790; Q561V9; Q6IAZ0; Q6IPY5 

Genbank Protein ID

 M22918; M22919; M22919; M22920; M31212; U02629; AB046613; CR457014; BC006781; BC017455; BC071661; BC093066 

Genbank Nucleotide ID

 AAA36347.1; AAA59892.1; AAA59893.1; AAA36348.1; AAA59853.1; AAA20643.1; BAB62402.1; CAG33295.1; AAH06781.2; AAH17455.1; AAH71661.1; AAH93066.1 

Protein Name

 Myosin light polypeptide 6 

Protein Synonyms/Alias

 17 kDa myosin light chain; LC17; Myosin light chain 3; MLC-3; Myosin light chain alkali 3; Myosin light chain A3; Smooth muscle and nonmuscle myosin light chain alkali 6 

Gene Name

 MYL6 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
26	FDRTGDGKILYSQCG	ubiquitination	[1, 2, 3]
50	PTNAEVLKVLGNPKS	ubiquitination	[1, 3, 4, 5]
56	LKVLGNPKSDEMNVK	ubiquitination	[1, 2]
63	KSDEMNVKVLDFEHF	ubiquitination	[1, 5]
79	PMLQTVAKNKDQGTY	ubiquitination	[1]
81	LQTVAKNKDQGTYED	acetylation	[6]
81	LQTVAKNKDQGTYED	ubiquitination	[1, 2, 3, 4, 5]
98	EGLRVFDKEGNGTVM	ubiquitination	[1]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Regulatory light chain of myosin. Does not bind calcium. 

Sequence Annotation

 DOMAIN 7 42 EF-hand 1.
 DOMAIN 84 119 EF-hand 2.
 DOMAIN 119 151 EF-hand 3.
 MOD_RES 2 2 N-acetylcysteine.
 MOD_RES 81 81 N6-acetyllysine.  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Motor protein; Muscle protein; Myosin; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 151 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016461; C:unconventional myosin complex; TAS:BHF-UCL.
 GO:0030898; F:actin-dependent ATPase activity; ISS:HGNC.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0003774; F:motor activity; TAS:HGNC.
 GO:0008307; F:structural constituent of muscle; IDA:HGNC.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0030049; P:muscle filament sliding; TAS:HGNC.
 GO:0007519; P:skeletal muscle tissue development; TAS:UniProtKB. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom. 

Pfam

 PF13405; EF_hand_4 

SMART

 SM00054; EFh 

PROSITE

 PS50222; EF_HAND_2 

PRINTS