CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023293
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytosolic carboxypeptidase 1 
Protein Synonyms/Alias
 ATP/GTP-binding protein 1; Nervous system nuclear protein induced by axotomy protein 1 homolog 
Gene Name
 AGTPBP1 
Gene Synonyms/Alias
 CCP1; KIAA1035; NNA1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10KLKVIPEKSLTNNSRubiquitination[1]
44TARYVTSKILHLAQSubiquitination[1, 2, 3]
232DTLAALLKSKTNARRubiquitination[3]
674PYGVQRTKIAQDIERubiquitination[1]
1007LQYLAAVKRLPLVYCubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5 (By similarity). 
Sequence Annotation
 ACT_SITE 970 970 Nucleophile (By similarity).
 METAL 920 920 Zinc (By similarity).
 METAL 923 923 Zinc (By similarity).
 METAL 1017 1017 Zinc (By similarity).  
Keyword
 Alternative splicing; Carboxypeptidase; Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Metalloprotease; Mitochondrion; Nucleus; Polymorphism; Protease; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1226 AA 
Protein Sequence
MSKLKVIPEK SLTNNSRIVG LLAQLEKINA EPSESDTARY VTSKILHLAQ SQEKTRREMT 60
AKGSTGMEIL LSTLENTKDL QTTLNILSIL VELVSAGGGR RVSFLVTKGG SQILLQLLMN 120
ASKESPPHED LMVQIHSILA KIGPKDKKFG VKARINGALN ITLNLVKQNL QNHRLVLPCL 180
QLLRVYSANS VNSVSLGKNG VVELMFKIIG PFSKKNSSLI KVALDTLAAL LKSKTNARRA 240
VDRGYVQVLL TIYVDWHRHD NRHRNMLIRK GILQSLKSVT NIKLGRKAFI DANGMKILYN 300
TSQECLAVRT LDPLVNTSSL IMRKCFPKNR LPLPTIKSSF HFQLPVIPVT GPVAQLYSLP 360
PEVDDVVDES DDNDDIDVEA ENETENEDDL DQNFKNDDIE TDINKLKPQQ EPGRTIEDLK 420
MYEHLFPELV DDFQDYDLIS KEPKPFVFEG KVRGPIVVPT AGEETSGNSG NLRKVVMKEN 480
ISSKGDEGEK KSTFMDLAKE DIKDNDRTLQ QQPGDQNRTI SSVHGLNNDI VKALDRITLQ 540
NIPSQTAPGF TAEMKKDCSL PLTVLTCAKA CPHMATCGNV LFEGRTVQLG KLCCTGVETE 600
DDEDTESNSS VEQASVEVPD GPTLHDPDLY IEIVKNTKSV PEYSEVAYPD YFGHIPPPFK 660
EPILERPYGV QRTKIAQDIE RLIHQSDIID RVVYDLDNPN YTIPEEGDIL KFNSKFESGN 720
LRKVIQIRKN EYDLILNSDI NSNHYHQWFY FEVSGMRPGV AYRFNIINCE KSNSQFNYGM 780
QPLMYSVQEA LNARPWWIRM GTDICYYKNH FSRSSVAAGG QKGKSYYTIT FTVNFPHKDD 840
VCYFAYHYPY TYSTLQMHLQ KLESAHNPQQ IYFRKDVLCE TLSGNSCPLV TITAMPESNY 900
YEHICHFRNR PYVFLSARVH PGETNASWVM KGTLEYLMSN NPTAQSLRES YIFKIVPMLN 960
PDGVINGNHR CSLSGEDLNR QWQSPSPDLH PTIYHAKGLL QYLAAVKRLP LVYCDYHGHS 1020
RKKNVFMYGC SIKETVWHTN DNATSCDVVE DTGYRTLPKI LSHIAPAFCM SSCSFVVEKS 1080
KESTARVVVW REIGVQRSYT MESTLCGCDQ GKYKGLQIGT RELEEMGAKF CVGLLRLKRL 1140
TSPLEYNLPS SLLDFENDLI ESSCKVTSPT TYVLDEDEPR FLEEVDYSAE SNDELDIELA 1200
ENVGDYEPSA QEEVLSDSEL SRTYLP 1226 
Gene Ontology
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004181; F:metallocarboxypeptidase activity; ISS:UniProtKB.
 GO:0015631; F:tubulin binding; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0007628; P:adult walking behavior; IEA:Compara.
 GO:0035609; P:C-terminal protein deglutamylation; ISS:UniProtKB.
 GO:0021702; P:cerebellar Purkinje cell differentiation; ISS:UniProtKB.
 GO:0001754; P:eye photoreceptor cell differentiation; ISS:UniProtKB.
 GO:0007005; P:mitochondrion organization; ISS:UniProtKB.
 GO:0050905; P:neuromuscular process; ISS:UniProtKB.
 GO:0042133; P:neurotransmitter metabolic process; IEA:Compara.
 GO:0021772; P:olfactory bulb development; ISS:UniProtKB.
 GO:0035610; P:protein side chain deglutamylation; ISS:UniProtKB.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0060041; P:retina development in camera-type eye; IEA:Compara. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000834; Peptidase_M14. 
Pfam
 PF00246; Peptidase_M14 
SMART
  
PROSITE
 PS00132; CARBOXYPEPT_ZN_1
 PS00133; CARBOXYPEPT_ZN_2 
PRINTS