CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006278
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin fusion degradation protein 4 
Protein Synonyms/Alias
 UB fusion protein 4 
Gene Name
 UFD4 
Gene Synonyms/Alias
 YKL010C; YKL162 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
174ARNERISKLIENTGNubiquitination[1]
349VEVLPTLKPIFSNATubiquitination[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). 
Sequence Annotation
 DOMAIN 1376 1483 HECT.
 REGION 1007 1081 K-box.
 ACT_SITE 1450 1450 Glycyl thioester intermediate (By
 MOD_RES 87 87 Phosphothreonine.  
Keyword
 Complete proteome; Ligase; Phosphoprotein; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1483 AA 
Protein Sequence
MSENNSHNLD EHESHSENSD YMMDTQVEDD YDEDGHVQGE YSYYPDEDED EHMLSSVGSF 60
EADDGEDDDN DYHHEDDSGL LYGYHRTQNG SDEDRNEEED GLERSHDNNE FGSNPLHLPD 120
ILETFAQRLE QRRQTSEGLG QHPVGRTLPE ILSMIGGRME RSAESSARNE RISKLIENTG 180
NASEDPYIAM ESLKELSENI LMMNQMVVDR IIPMETLIGN IAAILSDKIL REELELQMQA 240
CRCMYNLFEV CPESISIAVD EHVIPILQGK LVEISYIDLA EQVLETVEYI SRVHGRDILK 300
TGQLSIYVQF FDFLTIHAQR KAIAIVSNAC SSIRTDDFKT IVEVLPTLKP IFSNATDQPI 360
LTRLVNAMYG ICGALHGVDK FETLFSLDLI ERIVQLVSIQ DTPLENKLKC LDILTVLAMS 420
SDVLSRELRE KTDIVDMATR SFQHYSKSPN AGLHETLIYV PNSLLISISR FIVVLFPPED 480
ERILSADKYT GNSDRGVISN QEKFDSLVQC LIPILVEIYT NAADFDVRRY VLIALLRVVS 540
CINNSTAKAI NDQLIKLIGS ILAQKETASN ANGTYSSEAG TLLVGGLSLL DLICKKFSEL 600
FFPSIKREGI FDLVKDLSVD FNNIDLKEDG NENISLSDEE GDLHSSIEEC DEGDEEYDYE 660
FTDMEIPDSV KPKKISIHIF RTLSLAYIKN KGVNLVNRVL SQMNVEQEAI TEELHQIEGV 720
VSILENPSTP DKTEEDWKGI WSVLKKCIFH EDFDVSGFEF TSTGLASSIT KRITSSTVSH 780
FILAKSFLEV FEDCIDRFLE ILQSALTRLE NFSIVDCGLH DGGGVSSLAK EIKIKLVYDG 840
DASKDNIGTD LSSTIVSVHC IASFTSLNEF LRHRMVRMRF LNSLIPNLTS SSTEADREEE 900
ENCLDHMRKK NFDFFYDNEK VDMESTVFGV IFNTFVRRNR DLKTLWDDTH TIKFCKSLEG 960
NNRESEAAEE ANEGKKLRDF YKKREFAQVD TGSSADILTL LDFLHSCGVK SDSFINSKLS 1020
AKLARQLDEP LVVASGALPD WSLFLTRRFP FLFPFDTRML FLQCTSFGYG RLIQLWKNKS 1080
KGSKDLRNDE ALQQLGRITR RKLRISRKTI FATGLKILSK YGSSPDVLEI EYQEEAGTGL 1140
GPTLEFYSVV SKYFARKSLN MWRCNSYSYR SEMDVDTTDD YITTLLFPEP LNPFSNNEKV 1200
IELFGYLGTF VARSLLDNRI LDFRFSKVFF ELLHRMSTPN VTTVPSDVET CLLMIELVDP 1260
LLAKSLKYIV ANKDDNMTLE SLSLTFTVPG NDDIELIPGG CNKSLNSSNV EEYIHGVIDQ 1320
ILGKGIEKQL KAFIEGFSKV FSYERMLILF PDELVDIFGR VEEDWSMATL YTNLNAEHGY 1380
TMDSSIIHDF ISIISAFGKH ERRLFLQFLT GSPKLPIGGF KSLNPKFTVV LKHAEDGLTA 1440
DEYLPSVMTC ANYLKLPKYT SKDIMRSRLC QAIEEGAGAF LLS 1483 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0004842; F:ubiquitin-protein ligase activity; IBA:RefGenome.
 GO:0006513; P:protein monoubiquitination; TAS:SGD.
 GO:0000209; P:protein polyubiquitination; TAS:SGD.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IBA:RefGenome. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000569; HECT. 
Pfam
 PF00632; HECT 
SMART
 SM00119; HECTc 
PROSITE
 PS50237; HECT 
PRINTS