UniProt Accession

 OCTC_MOUSE; Q9DC50; Q921I4 

Genbank Protein ID

 AK004567; BC006593; BC012308 

Genbank Nucleotide ID

 BAB23378.1; AAH06593.1; AAH12308.1 

Protein Name

 Peroxisomal carnitine O-octanoyltransferase 

Protein Synonyms/Alias

 COT 

Gene Name

 Crot 

Gene Synonyms/Alias

 Cot 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
33	PALEESLKKYLESVK	ubiquitination	[1]
34	ALEESLKKYLESVKP	ubiquitination	[1]
40	KKYLESVKPFANEDE	acetylation	[2]
40	KKYLESVKPFANEDE	succinylation	[2]
40	KKYLESVKPFANEDE	ubiquitination	[1]
49	FANEDEYKKTEEIVQ	acetylation	[3]
50	ANEDEYKKTEEIVQK	ubiquitination	[1]
57	KTEEIVQKFQEGAGK	acetylation	[2]
57	KTEEIVQKFQEGAGK	succinylation	[2]
57	KTEEIVQKFQEGAGK	ubiquitination	[1]
69	AGKRLHQKLLERARG	ubiquitination	[1]
143	WQLLRREKLPVHKSG	acetylation	[4]
148	REKLPVHKSGNTPLD	ubiquitination	[1]
167	RMLFSTCKVPGITRD	ubiquitination	[1]
181	DSIMNYFKTESEGHC	acetylation	[2]
181	DSIMNYFKTESEGHC	succinylation	[2]
226	RQLTYIHKKCSNEPV	acetylation	[4]
227	QLTYIHKKCSNEPVG	ubiquitination	[1]
384	LNDVSQAKAQHLKAA	acetylation	[2]
384	LNDVSQAKAQHLKAA	succinylation	[2]
384	LNDVSQAKAQHLKAA	ubiquitination	[1]
406	STFTSFGKKLTKEEA	acetylation	[2, 4, 5]
406	STFTSFGKKLTKEEA	succinylation	[2]
406	STFTSFGKKLTKEEA	ubiquitination	[1]
482	SLLERQQKMLEAFAK	acetylation	[2]
482	SLLERQQKMLEAFAK	succinylation	[2]
482	SLLERQQKMLEAFAK	ubiquitination	[1]
489	KMLEAFAKHNKMMKD	ubiquitination	[1]
495	AKHNKMMKDCSHGKG	acetylation	[2, 3, 4, 6]
501	MKDCSHGKGFDRHLL	acetylation	[4]
501	MKDCSHGKGFDRHLL	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [5] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [6] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647] 

Functional Description

 Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate (By similarity). 

Sequence Annotation

 REGION 405 417 Coenzyme A binding (By similarity).
 MOTIF 610 612 Microbody targeting signal (Potential).
 ACT_SITE 327 327 Proton acceptor (By similarity).
 BINDING 439 439 Carnitine (By similarity).
 BINDING 442 442 Coenzyme A (By similarity).
 BINDING 452 452 Carnitine (By similarity).
 BINDING 505 505 Carnitine (By similarity).
 MOD_RES 495 495 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Acyltransferase; Complete proteome; Fatty acid metabolism; Lipid metabolism; Peroxisome; Reference proteome; Transferase; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 612 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005777; C:peroxisome; IDA:MGI.
 GO:0008458; F:carnitine O-octanoyltransferase activity; IDA:MGI.
 GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
 GO:0015908; P:fatty acid transport; IDA:MGI. 

Interpro

 IPR000542; Carn_acyl_trans. 

Pfam

 PF00755; Carn_acyltransf 

SMART

  

PROSITE

 PS00439; ACYLTRANSF_C_1
 PS00440; ACYLTRANSF_C_2 

PRINTS