| Tag | Content |
|---|
| CPLM ID | CPLM-008601 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Putative DNA helicase INO80 |
Protein Synonyms/Alias | Inositol-requiring protein 80 |
Gene Name | INO80 |
Gene Synonyms/Alias | YGL150C; G1880 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 9 | SLAVLLNKEDKDISD | acetylation | [1] | | 618 | SNDSESQKNIDISAL | acetylation | [1] | | 1439 | MRDRAKQKEQVQQVV | acetylation | [2] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] [2] Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures. Xie H, Bandhakavi S, Roe MR, Griffin TJ. J Proteome Res. 2007 May;6(5):2019-26. [ PMID: 17397211] |
Functional Description | Putative DNA helicase component of the INO80 complex which remodels chromatin by shifting nucleosomes. Its ability to induce transcription of some phosphate-responsive genes is modulated by inositol polyphosphates. The INO80 complex is involved in DNA repair by associating to 'Ser-129' phosphorylated H2A histones as a response to DNA damage. |
Sequence Annotation | DOMAIN 476 601 DBINO.
DOMAIN 718 890 Helicase ATP-binding.
DOMAIN 1303 1467 Helicase C-terminal.
NP_BIND 731 738 ATP (Potential).
MOTIF 841 844 DEAQ box.
MOD_RES 65 65 Phosphoserine.
MOD_RES 115 115 Phosphoserine.
MOD_RES 133 133 Phosphoserine.
MOD_RES 610 610 Phosphoserine. |
Keyword | Activator; ATP-binding; Chromatin regulator; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1489 AA |
Protein Sequence | MSLAVLLNKE DKDISDFSKT TAGKSAKKNS RERVADVAPT RVLDKKQAYL SQLNSEFNRI 60
KRRDSIEQLY QDWKFINLQE FELISEWNQQ SKDWQFDNTN DSQDLHFKKL YRDMSMINKE 120
WAEYQSFKNA NLSDIINEKD ADEDEEDDED ELEDGEEDME EDEASTGRHT NGKSMRGNGI 180
QKSRKKDAAA AAAIGKAIKD DQTHADTVVT VNGDENEDGN NGEDEDNDND NENNNDNDND 240
NENENDNDSD NDDEEENGEE DEEEEEIEDL DEEDFAAFEE QDDNDDEDFN PDVEKRRKRS 300
SSSSSSTKLS MNSLSLITSK KINKNITINS DRPKIVRELI KMCNKNKHQK IKKRRFTNCI 360
VTDYNPIDSK LNIKITLKQY HVKRLKKLIN DAKREREREE ALKNNVGLDG NDLDNDEDGS 420
ESHKRRKLNN NTANGADDAN KRKFNTRHGL PTYGMKMNAK EARAIQRHYD NTYTTIWKDM 480
ARKDSTKMSR LVQQIQSIRS TNFRKTSSLC AREAKKWQSK NFKQIKDFQT RARRGIREMS 540
NFWKKNEREE RDLKKKIEKE AMEQAKKEEE EKESKRQAKK LNFLLTQTEL YSHFIGRKIK 600
TNELEGNNVS SNDSESQKNI DISALAPNKN DFHAIDFDNE NDEQLRLRAA ENASNALAET 660
RAKAKQFDDH ANAHEEEEEE DELNFQNPTS LGEITIEQPK ILACTLKEYQ LKGLNWLANL 720
YDQGINGILA DEMGLGKTVQ SISVLAHLAE NHNIWGPFLV VTPASTLHNW VNEISKFLPQ 780
FKILPYWGNA NDRKVLRKFW DRKNLRYNKN APFHVMVTSY QMVVTDANYL QKMKWQYMIL 840
DEAQAIKSSQ SSRWKNLLSF HCRNRLLLTG TPIQNSMQEL WALLHFIMPS LFDSHDEFNE 900
WFSKDIESHA EANTKLNQQQ LRRLHMILKP FMLRRVKKNV QSELGDKIEI DVLCDLTQRQ 960
AKLYQVLKSQ ISTNYDAIEN AATNDSTSNS ASNSGSDQNL INAVMQFRKV CNHPDLFERA 1020
DVDSPFSFTT FGKTTSMLTA SVANNNSSVI SNSNMNLSSM SSNNISNGKF TDLIYSSRNP 1080
IKYSLPRLIY EDLILPNYNN DVDIANKLKN VKFNIFNPST NYELCLFLSK LTGEPSLNEF 1140
FRVSTTPLLK RVIERTNGPK NTDSLSFKTI TQELLEVTRN APSEGVMASL LNVEKHAYER 1200
EYLNCIQRGY HPNVSAPPVT IEVLGSSHVT NSINNELFDP LISQALSDIP AITQYNMHVK 1260
KGIPVEDFPK TGLFPEPLNK NFSSNISMPS MDRFITESAK LRKLDELLVK LKSEGHRVLI 1320
YFQMTKMMDL MEEYLTYRQY NHIRLDGSSK LEDRRDLVHD WQTNPEIFVF LLSTRAGGLG 1380
INLTAADTVI FYDSDWNPTI DSQAMDRAHR LGQTRQVTVY RLLVRGTIEE RMRDRAKQKE 1440
QVQQVVMEGK TQEKNIKTIE VGENDSEVTR EGSKSISQDG IKEAASALA 1489 |
Gene Ontology | GO:0031011; C:Ino80 complex; IDA:SGD. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0016887; F:ATPase activity; IDA:SGD. GO:0003677; F:DNA binding; IEA:UniProtKB-KW. GO:0004386; F:helicase activity; IEA:UniProtKB-KW. GO:0006348; P:chromatin silencing at telomere; IMP:SGD. GO:0006281; P:DNA repair; IDA:SGD. GO:0043486; P:histone exchange; IDA:SGD. GO:0042766; P:nucleosome mobilization; IMP:SGD. GO:0016584; P:nucleosome positioning; IDA:SGD. GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:SGD. GO:0043618; P:regulation of transcription from RNA polymerase II promoter in response to stress; IMP:SGD. GO:0006366; P:transcription from RNA polymerase II promoter; IDA:SGD. |
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