CPLM-020832

Genbank Nucleotide ID

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Protein Synonyms/Alias

NADH-ubiquinone oxidoreductase 24 kDa subunit

Mus musculus (Mouse)

Position	Peptide	Type	References
60	DFTPENYKRIEAIVK	acetylation	[1, 2, 3, 4, 5]
60	DFTPENYKRIEAIVK	ubiquitination	[6]
208	EEIIDELKAGKVPKP	acetylation	[1, 4, 5, 7]
208	EEIIDELKAGKVPKP	ubiquitination	[6]

[1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
[2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[6] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[7] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]

Functional Description

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

METAL 134 134 Iron-sulfur (2Fe-2S) (Potential).
METAL 139 139 Iron-sulfur (2Fe-2S) (Potential).
METAL 175 175 Iron-sulfur (2Fe-2S) (Potential).
METAL 179 179 Iron-sulfur (2Fe-2S) (Potential).
MOD_RES 192 192 Phosphotyrosine; by SRC (By similarity).

2Fe-2S; Alternative splicing; Complete proteome; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Respiratory chain; Transit peptide; Transport; Ubiquinone.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005747; C:mitochondrial respiratory chain complex I; IBA:RefGenome.
GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IBA:RefGenome.
GO:0048738; P:cardiac muscle tissue development; IEA:Compara.
GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IBA:RefGenome.
GO:0007399; P:nervous system development; IEA:Compara.

IPR002023; 2Fe-2S_Ferredox.
IPR012336; Thioredoxin-like_fold.

PF01257; Complex1_24kDa

PS01099; COMPLEX1_24K