UniProt Accession

 DAPB_ECOLI; P04036 

Genbank Protein ID

 M10611; U00096; AP009048; J01597 

Genbank Nucleotide ID

 AAA23666.1; AAC73142.1; BAB96600.1 

Protein Name

 4-hydroxy-tetrahydrodipicolinate reductase 

Protein Synonyms/Alias

 HTPA reductase 

Gene Name

 dapB 

Gene Synonyms/Alias

 b0031; JW0029 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
111	TGFDEAGKQAIRDAA	acetylation	[1]
163	IEAHHRHKVDAPSGT	acetylation	[1, 2]
184	AIAHALDKDLKDCAV	acetylation	[1]
187	HALDKDLKDCAVYSR	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Catalyzes the conversion of 4-hydroxy- tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate. Can use both NADH and NADPH as a reductant, with NADH being twice as effective as NADPH. 

Sequence Annotation

 NP_BIND 12 17 NAD(P).
 NP_BIND 102 104 NAD(P).
 NP_BIND 126 129 NAD(P).
 REGION 169 170 Substrate binding.
 ACT_SITE 159 159 Proton donor/acceptor.
 ACT_SITE 163 163 Proton donor.
 BINDING 38 38 NAD.
 BINDING 39 39 NADP.
 BINDING 160 160 Substrate.  

Keyword

 3D-structure; Amino-acid biosynthesis; Complete proteome; Cytoplasm; Diaminopimelate biosynthesis; Direct protein sequencing; Lysine biosynthesis; NAD; NADP; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 273 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0008839; F:4-hydroxy-tetrahydrodipicolinate reductase; IEA:EC.
 GO:0051287; F:NAD binding; IEA:HAMAP.
 GO:0070402; F:NADPH binding; IEA:InterPro.
 GO:0016726; F:oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor; IEA:HAMAP.
 GO:0019877; P:diaminopimelate biosynthetic process; IDA:EcoliWiki.
 GO:0009089; P:lysine biosynthetic process via diaminopimelate; IEA:HAMAP. 

Interpro

 IPR022663; DapB_C.
 IPR000846; DapB_N.
 IPR022664; DapB_N_CS.
 IPR011770; Dihydrodipicolinate_Rdtase.
 IPR023940; Dihydrodipicolinate_Rdtase_bac.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF05173; DapB_C
 PF01113; DapB_N 

SMART

  

PROSITE

 PS01298; DAPB 

PRINTS