UniProt Accession

 NIPA_HUMAN; Q86WB0; A6NH66; Q75MF3; Q75MF4; Q8N330; Q96F75; Q9HA34; Q9NVX4; Q9P0R0 

Genbank Protein ID

 AJ537494; AF151050; AK001317; AK022373; AC073320; AC073320; AC087071; BC011551; BC028917 

Genbank Nucleotide ID

 CAD61161.1; AAF36136.1; BAA91619.1; BAB14024.1; AAS07546.1; AAS07547.1; AAH11551.1; AAH28917.1 

Protein Name

 Nuclear-interacting partner of ALK 

Protein Synonyms/Alias

 Nuclear-interacting partner of anaplastic lymphoma kinase; hNIPA; Zinc finger C3HC-type protein 1 

Gene Name

 ZC3HC1 

Gene Synonyms/Alias

 NIPA; HSPC216 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
31	SPEGTPQKIRQLIDE	ubiquitination	[1]
75	PSLESTSKEAFFSRV	ubiquitination	[1, 2]
89	VETFSSLKWAGKPFE	ubiquitination	[1]
93	SSLKWAGKPFELSPL	ubiquitination	[1]
485	DSMSLSEKSRKVFRI	ubiquitination	[1, 2, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events. 

Sequence Annotation

 ZN_FING 102 156 C3HC-type.
 REGION 170 210 F-box-like.
 MOTIF 396 402 Nuclear localization signal.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 24 24 Phosphoserine.
 MOD_RES 28 28 Phosphothreonine.
 MOD_RES 58 58 Phosphoserine.
 MOD_RES 62 62 Phosphoserine.
 MOD_RES 321 321 Phosphoserine.
 MOD_RES 329 329 Phosphoserine.
 MOD_RES 333 333 Phosphothreonine.
 MOD_RES 335 335 Phosphoserine.
 MOD_RES 338 338 Phosphoserine.
 MOD_RES 344 344 Phosphoserine.
 MOD_RES 354 354 Phosphoserine.
 MOD_RES 359 359 Phosphoserine.
 MOD_RES 370 370 Phosphoserine.
 MOD_RES 384 384 Phosphothreonine.
 MOD_RES 387 387 Phosphothreonine.
 MOD_RES 395 395 Phosphoserine.  

Keyword

 Acetylation; Alternative splicing; Cell cycle; Cell division; Complete proteome; Metal-binding; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 502 AA 

Protein Sequence

Gene Ontology

 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway. 

Interpro

 IPR013909; NIPA/Rsm1.
 IPR012935; Znf_C3HC-like. 

Pfam

 PF08600; Rsm1
 PF07967; zf-C3HC 

SMART

  

PROSITE

  

PRINTS