CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005122
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ferrochelatase, mitochondrial 
Protein Synonyms/Alias
 Heme synthase; Protoheme ferro-lyase 
Gene Name
 FECH 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
37VCQPWRWKSGAAAAAacetylation[1]
106MTLPIQNKLAPFIAKubiquitination[2]
252LDHFPLEKRSEVVILubiquitination[3]
304YRLVWQSKVGPMPWLubiquitination[4]
415NPVCRETKSFFTSQQubiquitination[5]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Catalyzes the ferrous insertion into protoporphyrin IX. 
Sequence Annotation
 ACT_SITE 230 230
 ACT_SITE 383 383
 METAL 196 196 Iron-sulfur (2Fe-2S).
 METAL 403 403 Iron-sulfur (2Fe-2S).
 METAL 406 406 Iron-sulfur (2Fe-2S).
 METAL 411 411 Iron-sulfur (2Fe-2S).  
Keyword
 2Fe-2S; 3D-structure; Alternative splicing; Complete proteome; Disease mutation; Heme biosynthesis; Iron; Iron-sulfur; Lyase; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Polymorphism; Porphyrin biosynthesis; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 423 AA 
Protein Sequence
MRSLGANMAA ALRAAGVLLR DPLASSSWRV CQPWRWKSGA AAAAVTTETA QHAQGAKPQV 60
QPQKRKPKTG ILMLNMGGPE TLGDVHDFLL RLFLDRDLMT LPIQNKLAPF IAKRRTPKIQ 120
EQYRRIGGGS PIKIWTSKQG EGMVKLLDEL SPNTAPHKYY IGFRYVHPLT EEAIEEMERD 180
GLERAIAFTQ YPQYSCSTTG SSLNAIYRYY NQVGRKPTMK WSTIDRWPTH HLLIQCFADH 240
ILKELDHFPL EKRSEVVILF SAHSLPMSVV NRGDPYPQEV SATVQKVMER LEYCNPYRLV 300
WQSKVGPMPW LGPQTDESIK GLCERGRKNI LLVPIAFTSD HIETLYELDI EYSQVLAKEC 360
GVENIRRAES LNGNPLFSKA LADLVHSHIQ SNELCSKQLT LSCPLCVNPV CRETKSFFTS 420
QQL 423 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0004325; F:ferrochelatase activity; IDA:BHF-UCL.
 GO:0008198; F:ferrous iron binding; TAS:ProtInc.
 GO:0020037; F:heme binding; IEA:Compara.
 GO:0030350; F:iron-responsive element binding; IEA:Compara.
 GO:0008203; P:cholesterol metabolic process; IEA:Compara.
 GO:0009589; P:detection of UV; IEA:Compara.
 GO:0030218; P:erythrocyte differentiation; IEA:Compara.
 GO:0006091; P:generation of precursor metabolites and energy; TAS:ProtInc.
 GO:0006783; P:heme biosynthetic process; TAS:Reactome.
 GO:0055072; P:iron ion homeostasis; IEA:Compara.
 GO:0046501; P:protoporphyrinogen IX metabolic process; IDA:BHF-UCL.
 GO:0010999; P:regulation of eIF2 alpha phosphorylation by heme; IEA:Compara.
 GO:0046984; P:regulation of hemoglobin biosynthetic process; IEA:Compara.
 GO:0009416; P:response to light stimulus; TAS:ProtInc.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0034379; P:very-low-density lipoprotein particle assembly; IEA:Compara. 
Interpro
 IPR001015; Ferrochelatase.
 IPR019772; Ferrochelatase_AS. 
Pfam
 PF00762; Ferrochelatase 
SMART
  
PROSITE
 PS00534; FERROCHELATASE 
PRINTS