CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004420
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Integrin beta-4 
Protein Synonyms/Alias
 GP150; CD104 
Gene Name
 ITGB4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
173DYTIGFGKFVDKVSVubiquitination[1]
177GFGKFVDKVSVPQTDubiquitination[1]
191DMRPEKLKEPWPNSDubiquitination[1]
219DVDEFRNKLQGERISubiquitination[1]
388LRTEVTSKMFQKTRTubiquitination[1, 2]
437QKGNIHLKPSFSDGLubiquitination[1, 2]
656EECNFKVKMVDELKRubiquitination[2]
662VKMVDELKRAEEVVVubiquitination[1]
758RGHMVGFKEDHYMLRubiquitination[1, 2]
785MLRSGNLKGRDVVRWubiquitination[1, 2]
895LMAPRSAKPALLKLTubiquitination[1, 2]
904ALLKLTEKQVEQRAFubiquitination[1]
915QRAFHDLKVAPGYYTubiquitination[1, 2, 3]
958RPEDDDEKQLLVEAIubiquitination[1, 2]
1021RRVLDGGKSQVSYRTubiquitination[1]
1055FQPGEAWKELQVKLLubiquitination[1, 2]
1060AWKELQVKLLELQEVubiquitination[1, 2]
1160KPMGYRVKYWIQGDSubiquitination[2]
1713LSENVPYKFKVQARTubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. 
Sequence Annotation
 DOMAIN 29 73 PSI.
 DOMAIN 131 329 VWFA.
 REPEAT 456 502 I.
 REPEAT 503 542 II.
 REPEAT 543 581 III.
 REPEAT 582 619 IV.
 DOMAIN 979 1084 Calx-beta.
 DOMAIN 1126 1215 Fibronectin type-III 1.
 DOMAIN 1220 1318 Fibronectin type-III 2.
 DOMAIN 1528 1618 Fibronectin type-III 3.
 DOMAIN 1641 1734 Fibronectin type-III 4.
 REGION 456 619 Cysteine-rich tandem repeats.
 MOD_RES 1530 1530 Phosphothreonine.
 CARBOHYD 327 327 N-linked (GlcNAc...) (Potential).
 CARBOHYD 491 491 N-linked (GlcNAc...) (Potential).
 CARBOHYD 579 579 N-linked (GlcNAc...) (Potential).
 CARBOHYD 617 617 N-linked (GlcNAc...) (Potential).
 CARBOHYD 695 695 N-linked (GlcNAc...).
 DISULFID 30 455 By similarity.
 DISULFID 38 48 By similarity.
 DISULFID 41 72 By similarity.
 DISULFID 51 61 By similarity.
 DISULFID 245 288 By similarity.
 DISULFID 424 671 By similarity.
 DISULFID 452 457 By similarity.
 DISULFID 468 479 By similarity.
 DISULFID 476 512 By similarity.
 DISULFID 481 490 By similarity.
 DISULFID 492 503 By similarity.
 DISULFID 518 523 By similarity.
 DISULFID 520 551 By similarity.
 DISULFID 525 536 By similarity.
 DISULFID 557 562 By similarity.
 DISULFID 564 573 By similarity.
 DISULFID 575 582 By similarity.
 DISULFID 596 601 By similarity.
 DISULFID 598 648 By similarity.
 DISULFID 603 614 By similarity.
 DISULFID 626 635 By similarity.
 DISULFID 632 706 By similarity.
 DISULFID 651 680 By similarity.  
Keyword
 3D-structure; Alternative splicing; Cell adhesion; Cell junction; Complete proteome; Direct protein sequencing; Disease mutation; Disulfide bond; Epidermolysis bullosa; Glycoprotein; Integrin; Membrane; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1822 AA 
Protein Sequence
MAGPRPSPWA RLLLAALISV SLSGTLANRC KKAPVKSCTE CVRVDKDCAY CTDEMFRDRR 60
CNTQAELLAA GCQRESIVVM ESSFQITEET QIDTTLRRSQ MSPQGLRVRL RPGEERHFEL 120
EVFEPLESPV DLYILMDFSN SMSDDLDNLK KMGQNLARVL SQLTSDYTIG FGKFVDKVSV 180
PQTDMRPEKL KEPWPNSDPP FSFKNVISLT EDVDEFRNKL QGERISGNLD APEGGFDAIL 240
QTAVCTRDIG WRPDSTHLLV FSTESAFHYE ADGANVLAGI MSRNDERCHL DTTGTYTQYR 300
TQDYPSVPTL VRLLAKHNII PIFAVTNYSY SYYEKLHTYF PVSSLGVLQE DSSNIVELLE 360
EAFNRIRSNL DIRALDSPRG LRTEVTSKMF QKTRTGSFHI RRGEVGIYQV QLRALEHVDG 420
THVCQLPEDQ KGNIHLKPSF SDGLKMDAGI ICDVCTCELQ KEVRSARCSF NGDFVCGQCV 480
CSEGWSGQTC NCSTGSLSDI QPCLREGEDK PCSGRGECQC GHCVCYGEGR YEGQFCEYDN 540
FQCPRTSGFL CNDRGRCSMG QCVCEPGWTG PSCDCPLSNA TCIDSNGGIC NGRGHCECGR 600
CHCHQQSLYT DTICEINYSA IHPGLCEDLR SCVQCQAWGT GEKKGRTCEE CNFKVKMVDE 660
LKRAEEVVVR CSFRDEDDDC TYSYTMEGDG APGPNSTVLV HKKKDCPPGS FWWLIPLLLL 720
LLPLLALLLL LCWKYCACCK ACLALLPCCN RGHMVGFKED HYMLRENLMA SDHLDTPMLR 780
SGNLKGRDVV RWKVTNNMQR PGFATHAASI NPTELVPYGL SLRLARLCTE NLLKPDTREC 840
AQLRQEVEEN LNEVYRQISG VHKLQQTKFR QQPNAGKKQD HTIVDTVLMA PRSAKPALLK 900
LTEKQVEQRA FHDLKVAPGY YTLTADQDAR GMVEFQEGVE LVDVRVPLFI RPEDDDEKQL 960
LVEAIDVPAG TATLGRRLVN ITIIKEQARD VVSFEQPEFS VSRGDQVARI PVIRRVLDGG 1020
KSQVSYRTQD GTAQGNRDYI PVEGELLFQP GEAWKELQVK LLELQEVDSL LRGRQVRRFH 1080
VQLSNPKFGA HLGQPHSTTI IIRDPDELDR SFTSQMLSSQ PPPHGDLGAP QNPNAKAAGS 1140
RKIHFNWLPP SGKPMGYRVK YWIQGDSESE AHLLDSKVPS VELTNLYPYC DYEMKVCAYG 1200
AQGEGPYSSL VSCRTHQEVP SEPGRLAFNV VSSTVTQLSW AEPAETNGEI TAYEVCYGLV 1260
NDDNRPIGPM KKVLVDNPKN RMLLIENLRE SQPYRYTVKA RNGAGWGPER EAIINLATQP 1320
KRPMSIPIIP DIPIVDAQSG EDYDSFLMYS DDVLRSPSGS QRPSVSDDTG CGWKFEPLLG 1380
EELDLRRVTW RLPPELIPRL SASSGRSSDA EAPHGPPDDG GAGGKGGSLP RSATPGPPGE 1440
HLVNGRMDFA FPGSTNSLHR MTTTSAAAYG THLSPHVPHR VLSTSSTLTR DYNSLTRSEH 1500
SHSTTLPRDY STLTSVSSHD SRLTAGVPDT PTRLVFSALG PTSLRVSWQE PRCERPLQGY 1560
SVEYQLLNGG ELHRLNIPNP AQTSVVVEDL LPNHSYVFRV RAQSQEGWGR EREGVITIES 1620
QVHPQSPLCP LPGSAFTLST PSAPGPLVFT ALSPDSLQLS WERPRRPNGD IVGYLVTCEM 1680
AQGGGPATAF RVDGDSPESR LTVPGLSENV PYKFKVQART TEGFGPEREG IITIESQDGG 1740
PFPQLGSRAG LFQHPLQSEY SSITTTHTSA TEPFLVDGLT LGAQHLEAGG SLTRHVTQEF 1800
VSRTLTTSGT LSTHMDQQFF QT 1822 
Gene Ontology
 GO:0009925; C:basal plasma membrane; IEA:Compara.
 GO:0005604; C:basement membrane; IEA:Compara.
 GO:0031252; C:cell leading edge; IDA:UniProtKB.
 GO:0009986; C:cell surface; IDA:BHF-UCL.
 GO:0030056; C:hemidesmosome; IDA:UniProtKB.
 GO:0008305; C:integrin complex; TAS:ProtInc.
 GO:0004872; F:receptor activity; IEA:InterPro.
 GO:0007155; P:cell adhesion; NAS:ProtInc.
 GO:0048870; P:cell motility; IMP:UniProtKB.
 GO:0007160; P:cell-matrix adhesion; IEA:InterPro.
 GO:0030198; P:extracellular matrix organization; TAS:Reactome.
 GO:0046847; P:filopodium assembly; IEA:Compara.
 GO:0031581; P:hemidesmosome assembly; IDA:UniProtKB.
 GO:0007229; P:integrin-mediated signaling pathway; IEA:UniProtKB-KW.
 GO:0007275; P:multicellular organismal development; IEA:InterPro.
 GO:0009611; P:response to wounding; IDA:UniProtKB. 
Interpro
 IPR003644; Calx_beta.
 IPR013032; EGF-like_CS.
 IPR013111; EGF_extracell.
 IPR003961; Fibronectin_type3.
 IPR013783; Ig-like_fold.
 IPR015812; Integrin_bsu.
 IPR012013; Integrin_bsu-4.
 IPR002369; Integrin_bsu_N.
 IPR012896; Integrin_bsu_tail.
 IPR003659; Plexin-like.
 IPR016201; Plexin-like_fold.
 IPR002035; VWF_A. 
Pfam
 PF03160; Calx-beta
 PF07974; EGF_2
 PF00041; fn3
 PF07965; Integrin_B_tail
 PF00362; Integrin_beta 
SMART
 SM00237; Calx_beta
 SM00060; FN3
 SM00187; INB
 SM00423; PSI
 SM00327; VWA 
PROSITE
 PS00022; EGF_1
 PS01186; EGF_2
 PS50853; FN3
 PS00243; INTEGRIN_BETA
 PS50234; VWFA 
PRINTS
 PR01186; INTEGRINB.