CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007769
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphorylase b kinase regulatory subunit alpha, liver isoform 
Protein Synonyms/Alias
 Phosphorylase kinase alpha L subunit 
Gene Name
 PHKA2 
Gene Synonyms/Alias
 PHKLA; PYK 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
286TKNEIISKLQGRYGCubiquitination[1]
457HIKDLLRKHGVNVQSubiquitination[2]
682SYLPPLCKNTEDRHVubiquitination[1]
705DILSVMAKAKGLEVPubiquitination[2]
707LSVMAKAKGLEVPFVubiquitination[1, 2]
962LHHILSGKEFGVERSubiquitination[2]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin. 
Sequence Annotation
 REGION 807 837 Calmodulin-binding (Potential).
 REGION 1059 1099 Calmodulin-binding (Potential).
 MOD_RES 729 729 Phosphoserine.
 MOD_RES 735 735 Phosphoserine.
 MOD_RES 1015 1015 Phosphoserine.
 MOD_RES 1048 1048 Phosphothreonine (By similarity).
 LIPID 1232 1232 S-farnesyl cysteine (By similarity).  
Keyword
 Calmodulin-binding; Carbohydrate metabolism; Cell membrane; Complete proteome; Disease mutation; Glycogen metabolism; Glycogen storage disease; Lipoprotein; Membrane; Phosphoprotein; Polymorphism; Prenylation; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1235 AA 
Protein Sequence
MRSRSNSGVR LDGYARLVQQ TILCYQNPVT GLLSASHEQK DAWVRDNIYS ILAVWGLGMA 60
YRKNADRDED KAKAYELEQN VVKLMRGLLQ CMMRQVAKVE KFKHTQSTKD SLHAKYNTAT 120
CGTVVGDDQW GHLQVDATSL FLLFLAQMTA SGLRIIFTLD EVAFIQNLVF YIEAAYKVAD 180
YGMWERGDKT NQGIPELNAS SVGMAKAALE AIDELDLFGA HGGRKSVIHV LPDEVEHCQS 240
ILFSMLPRAS TSKEIDAGLL SIISFPAFAV EDVNLVNVTK NEIISKLQGR YGCCRFLRDG 300
YKTPREDPNR LHYDPAELKL FENIECEWPV FWTYFIIDGV FSGDAVQVQE YREALEGILI 360
RGKNGIRLVP ELYAVPPNKV DEEYKNPHTV DRVPMGKVPH LWGQSLYILS SLLAEGFLAA 420
GEIDPLNRRF STSVKPDVVV QVTVLAENNH IKDLLRKHGV NVQSIADIHP IQVQPGRILS 480
HIYAKLGRNK NMNLSGRPYR HIGVLGTSKL YVIRNQIFTF TPQFTDQHHF YLALDNEMIV 540
EMLRIELAYL CTCWRMTGRP TLTFPISRTM LTNDGSDIHS AVLSTIRKLE DGYFGGARVK 600
LGNLSEFLTT SFYTYLTFLD PDCDEKLFDN ASEGTFSPDS DSDLVGYLED TCNQESQDEL 660
DHYINHLLQS TSLRSYLPPL CKNTEDRHVF SAIHSTRDIL SVMAKAKGLE VPFVPMTLPT 720
KVLSAHRKSL NLVDSPQPLL EKVPESDFQW PRDDHGDVDC EKLVEQLKDC SNLQDQADIL 780
YILYVIKGPS WDTNLSGQHG VTVQNLLGEL YGKAGLNQEW GLIRYISGLL RKKVEVLAEA 840
CTDLLSHQKQ LTVGLPPEPR EKIISAPLPP EELTKLIYEA SGQDISIAVL TQEIVVYLAM 900
YVRAQPSLFV EMLRLRIGLI IQVMATELAR SLNCSGEEAS ESLMNLSPFD MKNLLHHILS 960
GKEFGVERSV RPIHSSTSSP TISIHEVGHT GVTKTERSGI NRLRSEMKQM TRRFSADEQF 1020
FSVGQAASSS AHSSKSARSS TPSSPTGTSS SDSGGHHIGW GERQGQWLRR RRLDGAINRV 1080
PVGFYQRVWK ILQKCHGLSI DGYVLPSSTT REMTPHEIKF AVHVESVLNR VPQPEYRQLL 1140
VEAIMVLTLL SDTEMTSIGG IIHVDQIVQM ASQLFLQDQV SIGAMDTLEK DQATGICHFF 1200
YDSAPSGAYG TMTYLTRAVA SYLQELLPNS GCQMQ 1235 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005964; C:phosphorylase kinase complex; TAS:ProtInc.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
 GO:0004689; F:phosphorylase kinase activity; TAS:ProtInc.
 GO:0006006; P:glucose metabolic process; TAS:Reactome.
 GO:0005980; P:glycogen catabolic process; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR008928; 6-hairpin_glycosidase-like.
 IPR012341; 6hp_glycosidase.
 IPR011613; Glyco_hydro_15.
 IPR008734; PHK_A/B_su. 
Pfam
 PF00723; Glyco_hydro_15 
SMART
  
PROSITE
  
PRINTS