CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002340
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Prothymosin alpha 
Protein Synonyms/Alias
 Prothymosin alpha, N-terminally processed; Thymosin alpha-1 
Gene Name
 PTMA 
Gene Synonyms/Alias
 TMSA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
15TSSEITTKDLKEKKEacetylation[1, 2]
15TSSEITTKDLKEKKEubiquitination[3, 4, 5, 6, 7, 8]
18EITTKDLKEKKEVVEacetylation[1]
20TTKDLKEKKEVVEEAacetylation[1]
20TTKDLKEKKEVVEEAubiquitination[9]
21TKDLKEKKEVVEEAEacetylation[1]
21TKDLKEKKEVVEEAEubiquitination[9]
103EDDDVDTKKQKTDEDacetylation[2]
103EDDDVDTKKQKTDEDubiquitination[3, 5, 8, 9]
Reference
 [1] RimJ is responsible for N(alpha)-acetylation of thymosin alpha1 in Escherichia coli.
 Fang H, Zhang X, Shen L, Si X, Ren Y, Dai H, Li S, Zhou C, Chen H.
 Appl Microbiol Biotechnol. 2009 Aug;84(1):99-104. [PMID: 19352641]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
 Prothymosin alpha may mediate immune function by conferring resistance to certain opportunistic infections. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 2 2 N-acetylserine; in Prothymosin alpha, N-
 MOD_RES 2 2 Phosphoserine.
 MOD_RES 8 8 Phosphothreonine (By similarity).
 MOD_RES 9 9 Phosphoserine.
 MOD_RES 10 10 Phosphoserine.
 MOD_RES 13 13 Phosphothreonine (By similarity).
 MOD_RES 14 14 Phosphothreonine (By similarity).
 MOD_RES 15 15 N6-acetyllysine.
 MOD_RES 102 102 Phosphothreonine.
 MOD_RES 107 107 Phosphothreonine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 111 AA 
Protein Sequence
MSDAAVDTSS EITTKDLKEK KEVVEEAENG RDAPANGNAE NEENGEQEAD NEVDEEEEEG 60
GEEEEEEEEG DGEEEDGDED EEAESATGKR AAEDDEDDDV DTKKQKTDED D 111 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0006351; P:transcription, DNA-dependent; TAS:ProtInc. 
Interpro
 IPR004931; Pro/parathymosin. 
Pfam
 PF03247; Prothymosin 
SMART
  
PROSITE
  
PRINTS