UniProt Accession

 SPOP_HUMAN; O43791; B2R6S3; D3DTW7; Q53HJ1 

Genbank Protein ID

 AJ000644; AK222589; AK312691; CH471109; CH471109; CH471109; CH471109; CH471109; BC001269; BC003385 

Genbank Nucleotide ID

 CAA04199.1; BAD96309.1; BAG35570.1; EAW94671.1; EAW94672.1; EAW94673.1; EAW94674.1; EAW94675.1; AAH01269.1; AAH03385.1 

Protein Name

 Speckle-type POZ protein 

Protein Synonyms/Alias

 HIB homolog 1; Roadkill homolog 1 

Gene Name

 SPOP 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
64	FSSGANDKLKWCLRV	ubiquitination	[1]
110	KFSILNAKGEETKAM	ubiquitination	[1, 2, 3, 4]
115	NAKGEETKAMESQRA	ubiquitination	[3]
215	GQEFQAHKAILAARS	ubiquitination	[1]
238	HEMEESKKNRVEIND	ubiquitination	[1]
279	DLLAAADKYALERLK	ubiquitination	[1, 2, 4, 5]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

 Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL. 

Sequence Annotation

 DOMAIN 31 161 MATH.
 DOMAIN 173 297 BTB.
 REGION 71 191 Required for nuclear localization.
 REGION 123 133 Important for binding substrate proteins.
 REGION 186 217 Important for homodimerization.
 REGION 297 355 Important for homodimerization.  

Keyword

 3D-structure; Complete proteome; Nucleus; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 374 AA 

Protein Sequence

Gene Ontology

 GO:0031463; C:Cul3-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IMP:UniProtKB.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway. 

Interpro

 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR002083; MATH.
 IPR008974; TRAF-like. 

Pfam

 PF00651; BTB
 PF00917; MATH 

SMART

 SM00225; BTB
 SM00061; MATH 

PROSITE

 PS50097; BTB
 PS50144; MATH 

PRINTS