CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030571
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2, isoform CRA_e 
Protein Synonyms/Alias
 Guanine nucleotide-binding protein G(i) subunit alpha-2; cDNA FLJ38980 fis, clone NT2RI2004884, highly similar to Guanine nucleotide-binding protein G(i), alpha-2 subunit; cDNA FLJ39276 fis, clone OCBBF2010843, highly similar to Guanine nucleotide-binding protein G(i), alpha-2 subunit 
Gene Name
 GNAI2 
Gene Synonyms/Alias
 hCG_1997820 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
38STIVKQMKIIHEDGYubiquitination[1, 2]
177VETHFTFKDLHFKMFubiquitination[1, 2, 3, 4]
182TFKDLHFKMFDVGGQubiquitination[1, 3, 4]
233NRMHESMKLFDSICNubiquitination[1, 2, 3]
256IILFLNKKDLFEEKIubiquitination[1]
280PEYTGANKYDEAASYubiquitination[1, 2, 5]
291AASYIQSKFEDLNKRubiquitination[3, 4]
297SKFEDLNKRKDTKEIubiquitination[1, 2]
299FEDLNKRKDTKEIYTubiquitination[1]
302LNKRKDTKEIYTHFTubiquitination[1, 2]
334VIIKNNLKDCGLF**ubiquitination[1, 2, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; GTP-binding; Nucleotide-binding; Reference proteome; Transducer. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 339 AA 
Protein Sequence
MTEGVKTLGW TKQKGGCHWG RSEGAGESGK STIVKQMKII HEDGYSEEEC RQYRAVVYSN 60
TIQSIMAIVK AMGNLQIDFA DPSRADDARQ LFALSCTAEE QGVLPDDLSG VIRRLWADHG 120
VQACFGRSRE YQLNDSAAYY LNDLERIAQS DYIPTQQDVL RTRVKTTGIV ETHFTFKDLH 180
FKMFDVGGQR SERKKWIHCF EGVTAIIFCV ALSAYDLVLA EDEEMNRMHE SMKLFDSICN 240
NKWFTDTSII LFLNKKDLFE EKITHSPLTI CFPEYTGANK YDEAASYIQS KFEDLNKRKD 300
TKEIYTHFTC ATDTKNVQFV FDAVTDVIIK NNLKDCGLF 339 
Gene Ontology
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0004871; F:signal transducer activity; IEA:UniProtKB-KW.
 GO:0007188; P:adenylate cyclase-modulating G-protein coupled receptor signaling pathway; IEA:InterPro. 
Interpro
 IPR001408; Gprotein_alpha_I.
 IPR001019; Gprotein_alpha_su.
 IPR011025; GproteinA_insert.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00503; G-alpha 
SMART
 SM00275; G_alpha 
PROSITE
  
PRINTS
 PR00318; GPROTEINA.
 PR00441; GPROTEINAI.