UniProt Accession

 SRSF6_HUMAN; Q13247; B7Z6J3; E1P5W6; Q13244; Q13245; Q96J06; Q9UJB8; Q9Y3N7 

Genbank Protein ID

 U30883; U30828; U30829; AK300411; AL031681; CH471077; CH471077; BC006832 

Genbank Nucleotide ID

 AAA93073.1; AAA93071.1; AAA93072.1; BAH13279.1; CAB43960.1; EAW75964.1; EAW75967.1; AAH06832.1 

Protein Name

 Serine/arginine-rich splicing factor 6 

Protein Synonyms/Alias

 Pre-mRNA-splicing factor SRP55; Splicing factor, arginine/serine-rich 6 

Gene Name

 SRSF6 

Gene Synonyms/Alias

 SFRS6; SRP55 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
101	RRTSGRDKYGPPVRT	acetylation	[1, 2, 3]
101	RRTSGRDKYGPPVRT	ubiquitination	[3, 4]
127	RCSWQDLKDFMRQAG	ubiquitination	[5, 6]
143	VTYADAHKERTNEGV	ubiquitination	[4]
165	DMKRALDKLDGTEIN	acetylation	[3, 7]
165	DMKRALDKLDGTEIN	ubiquitination	[3, 4, 5, 8, 9]
182	NIRLIEDKPRTSHRR	acetylation	[3]
242	RSRSKGRKSRSKSKS	methylation	[10]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [8] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [9] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [10] Mass spectrometry-based identification and characterisation of lysine and arginine methylation in the human proteome.
 Bremang M, Cuomo A, Agresta AM, Stugiewicz M, Spadotto V, Bonaldi T.
 Mol Biosyst. 2013 Jul 30;9(9):2231-47. [PMID: 23748837] 

Functional Description

 Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10. 

Sequence Annotation

 DOMAIN 1 72 RRM 1.
 DOMAIN 110 183 RRM 2.
 MOD_RES 45 45 Phosphoserine.
 MOD_RES 81 81 Phosphoserine.
 MOD_RES 84 84 Phosphoserine.
 MOD_RES 299 299 Phosphoserine.
 MOD_RES 303 303 Phosphoserine.
 MOD_RES 314 314 Phosphoserine.
 MOD_RES 316 316 Phosphoserine.  

Keyword

 Alternative splicing; Complete proteome; Direct protein sequencing; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Repressor; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 344 AA 

Protein Sequence

Gene Ontology

 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0031124; P:mRNA 3'-end processing; TAS:Reactome.
 GO:0006406; P:mRNA export from nucleus; TAS:Reactome.
 GO:0006376; P:mRNA splice site selection; TAS:ProtInc.
 GO:0048025; P:negative regulation of mRNA splicing, via spliceosome; IDA:UniProtKB.
 GO:0006369; P:termination of RNA polymerase II transcription; TAS:Reactome. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS