UniProt Accession

 I5P2_HUMAN; P32019; C9J6U5; Q5VSG9; Q5VSH0; Q5VSH1; Q658Q5; Q6P6D4; Q6PD53; Q86YE1 

Genbank Protein ID

 M74161; AL603790; AL929472; AL603790; AL929472; AL603790; AL929472; AL929472; AL603790; AL929472; AL603790; AL929472; AL603790; BX296560; BC042529; BC058932; AL833055 

Genbank Nucleotide ID

 AAA79207.1; CAH69926.1; CAH69926.1; CAH69928.1; CAH69928.1; CAH69929.1; CAH69929.1; CAH70076.1; CAH70076.1; CAH70079.1; CAH70079.1; CAH70080.1; CAH70080.1; AAH42529.2; AAH58932.1; CAH56301.1 

Protein Name

 Type II inositol 1,4,5-trisphosphate 5-phosphatase 

Protein Synonyms/Alias

 75 kDa inositol polyphosphate-5-phosphatase; Phosphoinositide 5-phosphatase; 5PTase 

Gene Name

 INPP5B 

Gene Synonyms/Alias

 OCRL2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
312	STITVSDKAHILSMQ	ubiquitination	[1, 2, 3]
360	NVNGQSPKECLRLWL	ubiquitination	[2]
404	PKEEEWFKAVSEGLH	ubiquitination	[1, 3]
496	ERRNQDYKDICSRMQ	ubiquitination	[2]
542	LDVEKVKKLIEEKDF	ubiquitination	[2]
547	VKKLIEEKDFQMLYA	ubiquitination	[2]
596	DDWDTSEKCRAPAWC	ubiquitination	[2, 4]
630	ALKTSDHKPVSSVFD	ubiquitination	[2]
650	VNDELYRKTLEEIVR	ubiquitination	[2]
674	IPSVSLSKREFCFQN	ubiquitination	[2, 5]
972	RNPAGHQKLDMTEKK	ubiquitination	[2, 5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events. 

Sequence Annotation

 DOMAIN 22 148 PH.
 DOMAIN 821 993 Rho-GAP.
 REGION 342 668 5-phosphatase (By similarity).
 REGION 459 460 Substrate binding.
 REGION 582 583 Substrate binding.
 REGION 596 598 Substrate binding.
 REGION 669 782 ASH (By similarity).
 METAL 355 355 Magnesium.
 METAL 383 383 Magnesium.
 BINDING 383 383 Substrate.
 MOD_RES 990 990 Cysteine methyl ester (Potential).
 LIPID 990 990 S-farnesyl cysteine (Potential).  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; Endosome; Golgi apparatus; Hydrolase; Lipoprotein; Magnesium; Membrane; Metal-binding; Methylation; Polymorphism; Prenylation; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 993 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:Compara.
 GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
 GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0052658; F:inositol-1,4,5-trisphosphate 5-phosphatase activity; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004439; F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity; IDA:UniProtKB.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0043647; P:inositol phosphate metabolic process; TAS:Reactome.
 GO:0046856; P:phosphatidylinositol dephosphorylation; IDA:UniProtKB.
 GO:0070613; P:regulation of protein processing; IEA:Compara.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome.
 GO:0030317; P:sperm motility; IEA:Compara.
 GO:0007283; P:spermatogenesis; IEA:Compara. 

Interpro

 IPR005135; Endo/exonuclease/phosphatase.
 IPR000300; IPPc.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom. 

Pfam

 PF03372; Exo_endo_phos
 PF00620; RhoGAP 

SMART

 SM00128; IPPc
 SM00324; RhoGAP 

PROSITE

 PS50003; PH_DOMAIN
 PS50238; RHOGAP 

PRINTS