CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017164
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitochondrial Rho GTPase 2 
Protein Synonyms/Alias
 MIRO-2; Ras homolog gene family member T2 
Gene Name
 Rhot2 
Gene Synonyms/Alias
 Arht2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
421QRSVLMCKVLGARGVubiquitination[1]
511VHCATIYKRYYMDGQacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
 Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (By similarity). 
Sequence Annotation
 DOMAIN 1 177 Miro 1.
 DOMAIN 184 219 EF-hand 1.
 DOMAIN 304 339 EF-hand 2.
 DOMAIN 411 620 Miro 2.
 NP_BIND 11 18 GTP 1 (Potential).
 NP_BIND 57 61 GTP 1 (Potential).
 NP_BIND 118 121 GTP 1 (Potential).
 NP_BIND 424 431 GTP 2 (Potential).
 NP_BIND 461 465 GTP 2 (Potential).
 NP_BIND 526 529 GTP 2 (Potential).  
Keyword
 Calcium; Complete proteome; GTP-binding; Hydrolase; Membrane; Metal-binding; Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding; Reference proteome; Repeat; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 620 AA 
Protein Sequence
MRRDVRILLL GEAQVGKTSL ILSLVGEEFP EEVPARAEEI TIPADVTPEK VPTHIVDYSE 60
AEQTEEELQE EIHKANVVCV VYDVSEETTI EKIRTKWIPL VNGRTATGPR LPIILVGNKS 120
DLRPGSTMEA VLPIMSQFPE IETCVECSAK HLRNISELFY YAQKAVLHPT APLYDPEAKQ 180
LRPACAQALT RIFRLSDQDR DHGLSDEELN AFQKSCFGHP LAPQALEDVK RVVCKNVSGG 240
VQNDRLTLEG FLFLNTLFIQ RGRHETTWTI LRRFGYSDSL ELTPDYLYPA LHVPPGCSTE 300
LNHRGYQFVQ RMFEKHDQDH DGVLSPTELQ NLFSVFSGAP WGPELLHTVP TQAGCLPLHG 360
YLCQWTLMTY LDVQQCLAHL GYLGYPTLCE QDSQAQAITV TREKKLDQEK GQTQRSVLMC 420
KVLGARGVGK SAFLQAFLGN SLGEARDPPE KFPLHTINTV RVNGQEKYLI LCEVNADSLL 480
DTSLDTTCDV ACLMFDSSDP KTFVHCATIY KRYYMDGQTP CLFIASKADL PEGVAPPGLS 540
PAEFCRRHRL PAPASFSCLG PAMPSTDVFT QLATMATFPH LVHTELHPTS FWLRGVLVAV 600
GTAVAAVLSF SLYRVLVKSR 620 
Gene Ontology
 GO:0031307; C:integral to mitochondrial outer membrane; ISS:UniProtKB.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0019725; P:cellular homeostasis; ISS:UniProtKB.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0097345; P:mitochondrial outer membrane permeabilization; ISS:UniProtKB.
 GO:0047497; P:mitochondrion transport along microtubule; ISS:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR013566; EF_hand_assoc_1.
 IPR013567; EF_hand_assoc_2.
 IPR002048; EF_hand_dom.
 IPR020860; MIRO.
 IPR013684; MIRO-like.
 IPR027417; P-loop_NTPase.
 IPR001806; Small_GTPase.
 IPR021181; Small_GTPase_Miro. 
Pfam
 PF08355; EF_assoc_1
 PF08356; EF_assoc_2
 PF13405; EF_hand_4
 PF08477; Miro 
SMART
 SM00054; EFh 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS51423; MIRO 
PRINTS
 PR00449; RASTRNSFRMNG.