CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017946
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pumilio homolog 2 
Protein Synonyms/Alias
 Pumilio-2 
Gene Name
 PUM2 
Gene Synonyms/Alias
 KIAA0235; PUMH2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
32WEPDDSTKDGQKGIFubiquitination[1]
596STSSDLYKRSSSSLAubiquitination[1, 2]
672AAPGAEAKYRSASSTubiquitination[2, 3]
747GSRFIQQKLERATPAubiquitination[1]
792EFGSLDQKLALATRIubiquitination[2]
964FASNVVEKCVTHASRubiquitination[1, 2]
1046HILAKLEKYYLKNSPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts. 
Sequence Annotation
 DOMAIN 706 1048 PUM-HD.
 REPEAT 726 761 Pumilio 1.
 REPEAT 762 797 Pumilio 2.
 REPEAT 798 835 Pumilio 3.
 REPEAT 836 871 Pumilio 4.
 REPEAT 872 907 Pumilio 5.
 REPEAT 908 943 Pumilio 6.
 REPEAT 944 979 Pumilio 7.
 REPEAT 983 1022 Pumilio 8.
 REGION 1 260 Interaction with SNAPIN.
 MOD_RES 82 82 Phosphoserine.
 MOD_RES 136 136 Phosphoserine.
 MOD_RES 178 178 Phosphoserine.
 MOD_RES 182 182 Phosphoserine.
 MOD_RES 184 184 Phosphothreonine.
 MOD_RES 587 587 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Phosphoprotein; Polymorphism; Reference proteome; Repeat; RNA-binding; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1066 AA 
Protein Sequence
MNHDFQALAL ESRGMGELLP TKKFWEPDDS TKDGQKGIFL GDDEWRETAW GASHHSMSQP 60
IMVQRRSGQG FHGNSEVNAI LSPRSESGGL GVSMVEYVLS SSPADKLDSR FRKGNFGTRD 120
AETDGPEKGD QKGKASPFEE DQNRDLKQGD DDDSKINGRG LPNGMDADCK DFNRTPGSRQ 180
ASPTEVVERL GPNTNPSEGL GPLPNPTANK PLVEEFSNPE TQNLDAMEQV GLESLQFDYP 240
GNQVPMDSSG ATVGLFDYNS QQQLFQRTNA LTVQQLTAAQ QQQYALAAAQ QPHIAGVFSA 300
GLAPAAFVPN PYIISAAPPG TDPYTAAGLA AAATLAGPAV VPPQYYGVPW GVYPANLFQQ 360
QAAAAANNTA SQQAASQAQP GQQQVLRAGA GQRPLTPNQG QQGQQAESLA AAAAANPTLA 420
FGQGLATGMP GYQVLAPTAY YDQTGALVVG PGARTGLGAP VRLMAPTPVL ISSAAAQAAA 480
AAAAGGTASS LTGSTNGLFR PIGTQPPQQQ QQQPSTNLQS NSFYGSSSLT NSSQSSSLFS 540
HGPGQPGSTS LGFGSGNSLG AAIGSALSGF GSSVGSSASS SATRRESLST SSDLYKRSSS 600
SLAPIGQPFY NSLGFSSSPS PIGMPLPSQT PGHSLTPPPS LSSHGSSSSL HLGGLTNGSG 660
RYISAAPGAE AKYRSASSTS SLFSSSSQLF PPSRLRYNRS DIMPSGRSRL LEDFRNNRFP 720
NLQLRDLIGH IVEFSQDQHG SRFIQQKLER ATPAERQMVF NEILQAAYQL MTDVFGNYVI 780
QKFFEFGSLD QKLALATRIR GHVLPLALQM YGCRVIQKAL ESISSDQQVI SEMVKELDGH 840
VLKCVKDQNG NHVVQKCIEC VQPQSLQFII DAFKGQVFVL STHPYGCRVI QRILEHCTAE 900
QTLPILEELH QHTEQLVQDQ YGNYVIQHVL EHGRPEDKSK IVSEIRGKVL ALSQHKFASN 960
VVEKCVTHAS RAERALLIDE VCCQNDGPHS ALYTMMKDQY ANYVVQKMID MAEPAQRKII 1020
MHKIRPHITT LRKYTYGKHI LAKLEKYYLK NSPDLGPIGG PPNGML 1066 
Gene Ontology
 GO:0010494; C:cytoplasmic stress granule; ISS:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0034063; P:stress granule assembly; IEA:Compara. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR001313; Pumilio_RNA-bd_rpt. 
Pfam
 PF00806; PUF 
SMART
 SM00025; Pumilio 
PROSITE
 PS50302; PUM
 PS50303; PUM_HD 
PRINTS