UniProt Accession

 COT1_YEAST; P32798; D6W314 

Genbank Protein ID

 M88252; X90565; Z75224; BK006948 

Genbank Nucleotide ID

 AAA74884.1; CAA62171.1; CAA99636.1; DAA11080.1 

Protein Name

 Cobalt uptake protein COT1 

Protein Synonyms/Alias

  

Gene Name

 COT1 

Gene Synonyms/Alias

 YOR316C; O6131 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
212	LTPENASKTPSYSTS	ubiquitination	[1]
301	PLSCKASKILLQATP	ubiquitination	[1, 2, 3, 4]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [3] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [4] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 Probably responsible for the uptake of cobalt ions. It appears to act in a dosage-dependent manner to counteract the adverse effects of cobalt ions on cells. It may participate in the regulation of cobalt levels under normal physiological conditions and may be important in the supply of metal that is required for metalloenzyme or cofactor synthesis. It reduces the toxicity of cobalt and rhodium ions. Other components responsible for cobalt transport exist. 

Sequence Annotation

 MOD_RES 225 225 Phosphoserine.
 CROSSLNK 301 301 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Cobalt; Complete proteome; Isopeptide bond; Membrane; Mitochondrion; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 439 AA 

Protein Sequence

Gene Ontology

 GO:0000324; C:fungal-type vacuole; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0015087; F:cobalt ion transmembrane transporter activity; IMP:SGD.
 GO:0005385; F:zinc ion transmembrane transporter activity; IGI:SGD.
 GO:0006882; P:cellular zinc ion homeostasis; IMP:SGD. 

Interpro

 IPR002524; Cation_efflux.
 IPR027469; Cation_efflux_TMD. 

Pfam

 PF01545; Cation_efflux 

SMART

  

PROSITE

  

PRINTS