CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023182
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TBC1 domain family member 24 
Protein Synonyms/Alias
  
Gene Name
 TBC1D24 
Gene Synonyms/Alias
 KIAA1171 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
36CTELQELKQLARQGYubiquitination[1]
80VYSDIVGKIVGKHSSubiquitination[2, 3, 4]
84IVGKIVGKHSSSCLPubiquitination[1]
266PLESDSVKQDIRTFVubiquitination[1, 3]
288SPEKLLEKAFAIRLFacetylation[5]
288SPEKLLEKAFAIRLFubiquitination[1]
298AIRLFSRKEIQLLQMubiquitination[1]
312MANEKALKQKGITVKacetylation[5]
314NEKALKQKGITVKQKacetylation[5]
319KQKGITVKQKSVSLSacetylation[5]
454IKHPELTKPPPLMAAubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861
Functional Description
 May act as a GTPase-activating protein for Rab family protein(s). Involved in neuronal projections development, probably through a negative modulation of ARF6 function. 
Sequence Annotation
 DOMAIN 47 262 Rab-GAP TBC.
 DOMAIN 368 554 TLD.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Disease mutation; Epilepsy; GTPase activation; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 559 AA 
Protein Sequence
MDSPGYNCFV DKDKMDAAIQ DLGPKELSCT ELQELKQLAR QGYWAQSHAL RGKVYQRLIR 60
DIPCRTVTPD ASVYSDIVGK IVGKHSSSCL PLPEFVDNTQ VPSYCLNARG EGAVRKILLC 120
LANQFPDISF CPALPAVVAL LLHYSIDEAE CFEKACRILA CNDPGRRLID QSFLAFESSC 180
MTFGDLVNKY CQAAHKLMVA VSEDVLQVYA DWQRWLFGEL PLCYFARVFD VFLVEGYKVL 240
YRVALAILKF FHKVRAGQPL ESDSVKQDIR TFVRDIAKTV SPEKLLEKAF AIRLFSRKEI 300
QLLQMANEKA LKQKGITVKQ KSVSLSKRQF VHLAVHAENF RSEIVSVREM RDIWSWVPER 360
FALCQPLLLF SSLQHGYSLA RFYFQCEGHE PTLLLIKTTQ KEVCGAYLST DWSERNKFGG 420
KLGFFGTGEC FVFRLQPEVQ RYEWVVIKHP ELTKPPPLMA AEPTAPLSHS ASSDPADRLS 480
PFLAARHFNL PSKTESMFMA GGSDCLIVGG GGGQALYIDG DLNRGRTSHC DTFNNQPLCS 540
ENFLIAAVEA WGFQDPDTQ 559 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005097; F:Rab GTPase activator activity; IEA:InterPro.
 GO:0031175; P:neuron projection development; IMP:UniProtKB.
 GO:0032851; P:positive regulation of Rab GTPase activity; IEA:GOC. 
Interpro
 IPR000195; Rab-GTPase-TBC_dom.
 IPR006571; TLDc. 
Pfam
 PF00566; RabGAP-TBC
 PF07534; TLD 
SMART
 SM00164; TBC
 SM00584; TLDc 
PROSITE
 PS50086; TBC_RABGAP 
PRINTS