CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001730
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytochrome P450 1A2 
Protein Synonyms/Alias
 CYPIA2; Cytochrome P450-P2; Cytochrome P450-P3 
Gene Name
 Cyp1a2 
Gene Synonyms/Alias
 Cyp1a-2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
144RLAQDALKSFSIASDubiquitination[1]
166YLEEHVSKEANHLVSubiquitination[1]
174EANHLVSKLQKAMAEubiquitination[1]
211FGKNFPRKSEEMLNIubiquitination[1]
250YLPNPALKRFKTFNDubiquitination[1]
253NPALKRFKTFNDNFVubiquitination[1]
276EHYQDFNKNSIQDITubiquitination[1]
288DITSALFKHSENYKDacetylation[2]
288DITSALFKHSENYKDubiquitination[1]
294FKHSENYKDNGGLIPubiquitination[1]
340TWPNVQRKIHEELDTubiquitination[1]
401LNGFHIPKERCIYINubiquitination[1]
417WQVNHDEKQWKDPFVubiquitination[1]
420NHDEKQWKDPFVFRPubiquitination[1]
440NNNSAIDKTQSEKVMacetylation[3]
440NNNSAIDKTQSEKVMubiquitination[1]
445IDKTQSEKVMLFGLGubiquitination[1]
453VMLFGLGKRRCIGEIubiquitination[1]
498PNYGLTMKPGTCEHVubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
 Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation (By similarity). 
Sequence Annotation
 METAL 456 456 Iron (heme axial ligand).
 BINDING 225 225 Substrate (By similarity).
 MOD_RES 288 288 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Monooxygenase; Oxidoreductase; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 513 AA 
Protein Sequence
MAFSQYISLA PELLLATAIF CLVFWMVRAS RTQVPKGLKN PPGPWGLPFI GHMLTVGKNP 60
HLSLTRLSQQ YGDVLQIRIG STPVVVLSGL NTIKQALVRQ GDDFKGRPDL YSFTLITNGK 120
SMTFNPDSGP VWAARRRLAQ DALKSFSIAS DPTSASSCYL EEHVSKEANH LVSKLQKAMA 180
EVGHFEPVSQ VVESVANVIG AMCFGKNFPR KSEEMLNIVN NSKDFVENVT SGNAVDFFPV 240
LRYLPNPALK RFKTFNDNFV LFLQKTVQEH YQDFNKNSIQ DITSALFKHS ENYKDNGGLI 300
PEEKIVNIVN DIFGAGFDTV TTAITWSILL LVTWPNVQRK IHEELDTVVG RDRQPRLSDR 360
PQLPYLEAFI LEIYRYTSFV PFTIPHSTTR DTSLNGFHIP KERCIYINQW QVNHDEKQWK 420
DPFVFRPERF LTNNNSAIDK TQSEKVMLFG LGKRRCIGEI PAKWEVFLFL AILLQHLEFS 480
VPPGVKVDLT PNYGLTMKPG TCEHVQAWPR FSK 513 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0070330; F:aromatase activity; IEA:EC.
 GO:0034875; F:caffeine oxidase activity; IEA:Compara.
 GO:0032451; F:demethylase activity; IEA:Compara.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0020037; F:heme binding; ISS:UniProtKB.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0016712; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; IDA:MGI.
 GO:0009820; P:alkaloid metabolic process; IEA:Compara.
 GO:0045333; P:cellular respiration; IMP:MGI.
 GO:0071276; P:cellular response to cadmium ion; IDA:MGI.
 GO:0018894; P:dibenzo-p-dioxin metabolic process; IMP:MGI.
 GO:0017144; P:drug metabolic process; IMP:MGI.
 GO:0042738; P:exogenous drug catabolic process; IEA:Compara.
 GO:0050665; P:hydrogen peroxide biosynthetic process; IMP:MGI.
 GO:0030324; P:lung development; IMP:MGI.
 GO:0032787; P:monocarboxylic acid metabolic process; IEA:Compara.
 GO:0016098; P:monoterpenoid metabolic process; IEA:Compara.
 GO:0071615; P:oxidative deethylation; IEA:Compara.
 GO:0070989; P:oxidative demethylation; IEA:Compara.
 GO:0006778; P:porphyrin-containing compound metabolic process; IMP:MGI.
 GO:0009791; P:post-embryonic development; IMP:MGI.
 GO:0010468; P:regulation of gene expression; IMP:MGI.
 GO:0032355; P:response to estradiol stimulus; IEA:Compara.
 GO:0032496; P:response to lipopolysaccharide; IEA:Compara.
 GO:0006950; P:response to stress; IEA:Compara.
 GO:0006706; P:steroid catabolic process; IEA:Compara.
 GO:0009403; P:toxin biosynthetic process; IEA:Compara.
 GO:0009404; P:toxin metabolic process; IMP:MGI. 
Interpro
 IPR001128; Cyt_P450.
 IPR017972; Cyt_P450_CS.
 IPR002401; Cyt_P450_E_grp-I.
 IPR008066; Cyt_P450_E_grp-I_CYP1. 
Pfam
 PF00067; p450 
SMART
  
PROSITE
 PS00086; CYTOCHROME_P450 
PRINTS
 PR00463; EP450I.
 PR01683; EP450ICYP1A.
 PR00385; P450.