UniProt Accession

 ELAV1_HUMAN; Q15717; Q53XN6; Q9BTT1 

Genbank Protein ID

 U38175; BT009793; CH471139; BC003376 

Genbank Nucleotide ID

 AAB41913.1; AAP88795.1; EAW68949.1; AAH03376.1 

Protein Name

 ELAV-like protein 1 

Protein Synonyms/Alias

 Hu-antigen R; HuR 

Gene Name

 ELAVL1 

Gene Synonyms/Alias

 HUR 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
50	IGEVESAKLIRDKVA	ubiquitination	[1, 2, 3, 4]
55	SAKLIRDKVAGHSLG	ubiquitination	[1, 3]
72	FVNYVTAKDAERAIN	ubiquitination	[1, 3, 4]
92	RLQSKTIKVSYARPS	ubiquitination	[1]
104	RPSSEVIKDANLYIS	ubiquitination	[1, 3, 4]
120	LPRTMTQKDVEDMFS	ubiquitination	[1, 3]
182	SSEPITVKFAANPNQ	ubiquitination	[3]
191	AANPNQNKNVALLSQ	ubiquitination	[1, 2, 3, 4, 5]
283	IRDFNTNKCKGFGFV	ubiquitination	[3]
313	NGYRLGDKILQVSFK	ubiquitination	[1, 3]
320	KILQVSFKTNKSHK*	ubiquitination	[1, 3, 4]
323	QVSFKTNKSHK****	ubiquitination	[3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048] 

Functional Description

 Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'- UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA. 

Sequence Annotation

 DOMAIN 20 98 RRM 1.
 DOMAIN 106 186 RRM 2.
 DOMAIN 244 322 RRM 3.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.
 MOD_RES 202 202 Phosphoserine.
 MOD_RES 217 217 Omega-N-methylated arginine; by CARM1.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Methylation; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 326 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0035925; F:mRNA 3'-UTR AU-rich region binding; IDA:MGI.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0070935; P:3'-UTR-mediated mRNA stabilization; IDA:UniProtKB.
 GO:0010467; P:gene expression; TAS:Reactome.
 GO:0016071; P:mRNA metabolic process; TAS:Reactome.
 GO:0007275; P:multicellular organismal development; TAS:ProtInc.
 GO:0045727; P:positive regulation of translation; IDA:MGI. 

Interpro

 IPR006548; ELAD_HUD_SF.
 IPR002343; Hud_Sxl_RNA.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS

 PR00961; HUDSXLRNA.