CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005521
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NAD-dependent malic enzyme 
Protein Synonyms/Alias
 NAD-ME 
Gene Name
 maeA 
Gene Synonyms/Alias
 sfcA; b1479; JW5238 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
68WIQYQGFKTEIDKHIacetylation[1]
73GFKTEIDKHIYLRNIacetylation[1]
331SEEAARQKVFMVDRFacetylation[1]
344RFGLLTDKMPNLLPFacetylation[1]
354NLLPFQTKLVQKRENacetylation[1]
528AIAFAVGKMAQQQGVacetylation[1]
538QQQGVAVKTSAEALQacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
  
Sequence Annotation
 ACT_SITE 104 104 Proton donor (By similarity).
 ACT_SITE 175 175 Proton acceptor (By similarity).
 METAL 246 246 Divalent metal cation (By similarity).
 METAL 247 247 Divalent metal cation (By similarity).
 METAL 270 270 Divalent metal cation (By similarity).
 BINDING 157 157 NAD (By similarity).
 BINDING 270 270 NAD (By similarity).
 BINDING 418 418 NAD (By similarity).  
Keyword
 Complete proteome; Metal-binding; NAD; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 565 AA 
Protein Sequence
MEPKTKKQRS LYIPYAGPVL LEFPLLNKGS AFSMEERRNF NLLGLLPEVV ETIEEQAERA 60
WIQYQGFKTE IDKHIYLRNI QDTNETLFYR LVNNHLDEMM PVIYTPTVGA ACERFSEIYR 120
RSRGVFISYQ NRHNMDDILQ NVPNHNIKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT 180
ACGGISPAYT LPVVLDVGTN NQQLLNDPLY MGWRNPRITD DEYYEFVDEF IQAVKQRWPD 240
VLLQFEDFAQ KNAMPLLNRY RNEICSFNDD IQGTAAVTVG TLIAASRAAG GQLSEKKIVF 300
LGAGSAGCGI AEMIISQTQR EGLSEEAARQ KVFMVDRFGL LTDKMPNLLP FQTKLVQKRE 360
NLSDWDTDSD VLSLLDVVRN VKPDILIGVS GQTGLFTEEI IREMHKHCPR PIVMPLSNPT 420
SRVEATPQDI IAWTEGNALV ATGSPFNPVV WKDKIYPIAQ CNNAFIFPGI GLGVIASGAS 480
RITDEMLMSA SETLAQYSPL VLNGEGMVLP ELKDIQKVSR AIAFAVGKMA QQQGVAVKTS 540
AEALQQAIDD NFWQAEYRDY RRTSI 565 
Gene Ontology
 GO:0016619; F:malate dehydrogenase (oxaloacetate-decarboxylating) activity; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0006094; P:gluconeogenesis; IMP:EcoCyc.
 GO:0006108; P:malate metabolic process; IEA:InterPro. 
Interpro
 IPR015884; Malic_enzyme_CS.
 IPR012301; Malic_N.
 IPR012302; Malic_NAD-bd.
 IPR001891; Malic_OxRdtase.
 IPR016040; NAD(P)-bd_dom.
 IPR023667; NAD_malic_enz_proteobac. 
Pfam
 PF00390; malic
 PF03949; Malic_M 
SMART
 SM00919; Malic_M 
PROSITE
 PS00331; MALIC_ENZYMES 
PRINTS
 PR00072; MALOXRDTASE.