UniProt Accession

 EIF3L_HUMAN; Q9Y262; B2RDG6; B4DYB2; G8JLH4; Q53HQ1; Q53HT4; Q5QTR1; Q5TI15; Q6ICD2 

Genbank Protein ID

 AF077207; AF083243; AF109359; CR456436; AK302346; AK315533; AK222496; AK222529; Z97630; AL022311; CH471095; BC001101; BC007510; BC029265 

Genbank Nucleotide ID

 AAD27002.1; AAD39841.1; AAQ13507.1; CAG30322.1; BAG63674.1; BAG37913.1; BAD96216.1; BAD96249.1; CAX14899.1; CAX14899.1; EAW60198.1; AAH01101.1; AAH07510.1; AAH29265.1 

Protein Name

 Eukaryotic translation initiation factor 3 subunit L 

Protein Synonyms/Alias

 eIF3l; Eukaryotic translation initiation factor 3 subunit 6-interacting protein; Eukaryotic translation initiation factor 3 subunit E-interacting protein 

Gene Name

 EIF3L 

Gene Synonyms/Alias

 EIF3EIP; EIF3S6IP; HSPC021; HSPC025; MSTP005 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
70	VSDLIDQKVYELQAS	ubiquitination	[1]
87	SSDVIDQKVYEIQDI	ubiquitination	[1]
101	IYENSWTKLTERFFK	ubiquitination	[1, 2, 3]
143	YYRHIYAKVSGGPSL	ubiquitination	[1]
301	LENIELNKKSMYSRV	ubiquitination	[1]
356	MFQRTTYKYEMINKQ	ubiquitination	[1]
419	LFSYSCPKFLSPVVP	ubiquitination	[2]
437	NVHPNYHKEPFLQQL	acetylation	[4]
437	NVHPNYHKEPFLQQL	ubiquitination	[1, 2]
465	STIRSFLKLYTTMPV	acetylation	[4]
465	STIRSFLKLYTTMPV	ubiquitination	[1]
494	RIQLLVFKHKMKNLV	ubiquitination	[1, 2]
534	MIHIADTKVARRYGD	ubiquitination	[1]
549	FFIRQIHKFEELNRT	acetylation	[4]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. 

Sequence Annotation

 MOD_RES 2 2 N-acetylserine.
 MOD_RES 465 465 N6-acetyllysine.
 MOD_RES 549 549 N6-acetyllysine.  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Initiation factor; Polymorphism; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 564 AA 

Protein Sequence

Gene Ontology

 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IDA:UniProtKB.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP.
 GO:0006413; P:translational initiation; IDA:UniProtKB. 

Interpro

 IPR019382; eIF3l. 

Pfam

 PF10255; Paf67 

SMART

  

PROSITE

  

PRINTS