CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036854
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 DNA repair protein RAD50 
Protein Synonyms/Alias
  
Gene Name
 RAD50 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
6**MSRIEKMSILGVRubiquitination[1]
20RSFGIEDKDKQIITFmethylation[2]
62GDFPPGTKGNTFVHDubiquitination[3]
211KEYQMELKYLKQYKEubiquitination[3]
230IRDQITSKEAQLTSSubiquitination[4]
242TSSKEIVKSYENELDubiquitination[4]
256DPLKNRLKEIEHNLSubiquitination[1]
264EIEHNLSKIMKLDNEacetylation[5]
295ELEEKMEKVFQGTDEubiquitination[3]
461QNELKNVKYELQQLEubiquitination[3]
524DWLHSKSKEINQTRDacetylation[6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 660 AA 
Protein Sequence
MSRIEKMSIL GVRSFGIEDK DKQIITFFSP LTILVGPNGA GKTTIIECLK YICTGDFPPG 60
TKGNTFVHDP KVAQETDVRA QIRLQFRDVN GELIAVQRSM VCTQKSKKTE FKTLEGVITR 120
TKHGEKVSLS SKCAEIDREM ISSLGVSKAV LNNVIFCHQE DSNWPLSEGK ALKQKFDEIF 180
SATRYIKALE TLRQVRQTQG QKVKEYQMEL KYLKQYKEKA CEIRDQITSK EAQLTSSKEI 240
VKSYENELDP LKNRLKEIEH NLSKIMKLDN EIKALDSRKK QMEKDNSELE EKMEKVFQGT 300
DEQLNDLYHN HQRTVREKER KLVDCHRELE KLNKESRLLN QEKSELLVEQ GRLQLQADRH 360
QEHIRARDSL IQSLATQLEL DGFERGPFSE RQIKNFHKLV RERQEGEAKT ANQLMNDFAE 420
KETLKQKQID EIRDKKTGLG RIIELKSEIL SKKQNELKNV KYELQQLEGS SDRILELDQE 480
LIKAADKDEQ IRKIKSRHSD ELTSLLGYFP NKKQLEDWLH SKSKEINQTR DRLAKLNKEL 540
ASSEQNKNHI NNELKRKEEQ LSSYEDKLFD VCGSQDFESD LDRLKEEIEK SSKQRAMLAG 600
ATAVYSQFIT QLTDENQSCC PVCQRVFQTE AELQEVISDL QSKLRLAPDK LKSTESELKK 660 
Gene Ontology
 GO:0030870; C:Mre11 complex; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004518; F:nuclease activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006281; P:DNA repair; IEA:InterPro.
 GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR004584; Rad50.
 IPR007517; Rad50_Zn_hook.
 IPR013134; Zn_hook_Rad50. 
Pfam
 PF04423; Rad50_zn_hook 
SMART
  
PROSITE
 PS51131; ZN_HOOK 
PRINTS