CPLM-022516

Genbank Nucleotide ID

Serine/threonine-protein kinase pim-2

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
40	RLGPLLGKGGFGTVF	ubiquitination	[1, 2]
61	DRLQVAIKVIPRNRV	ubiquitination	[1]
89	LEVALLWKVGAGGGH	ubiquitination	[1]
165	GVVHRDIKDENILID	ubiquitination	[1, 2]
179	DLRRGCAKLIDFGSG	ubiquitination	[1]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti- apoptotic protein Bcl-X(L)/BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Isoform 1 is less active in this respect. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.

DOMAIN 32 286 Protein kinase.
NP_BIND 38 46 ATP (By similarity).
ACT_SITE 163 163 Proton acceptor (By similarity).
BINDING 61 61 ATP (By similarity).

3D-structure; Apoptosis; ATP-binding; Cell cycle; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Serine/threonine-protein kinase; Transferase.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
GO:0008637; P:apoptotic mitochondrial changes; IEA:Compara.
GO:0008283; P:cell proliferation; TAS:ProtInc.
GO:0000082; P:G1/S transition of mitotic cell cycle; IDA:UniProtKB.
GO:0007140; P:male meiosis; TAS:ProtInc.
GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
GO:0008285; P:negative regulation of cell proliferation; IDA:UniProtKB.
GO:0010508; P:positive regulation of autophagy; ISS:UniProtKB.
GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; ISS:UniProtKB.
GO:0045893; P:positive regulation of transcription, DNA-dependent; ISS:UniProtKB.
GO:0050821; P:protein stabilization; IMP:UniProtKB.
GO:0009615; P:response to virus; IEP:UniProtKB.

IPR011009; Kinase-like_dom.
IPR000719; Prot_kinase_cat_dom.
IPR017441; Protein_kinase_ATP_BS.
IPR002290; Ser/Thr_dual-sp_kinase_dom.
IPR008271; Ser/Thr_kinase_AS.

PS00107; PROTEIN_KINASE_ATP
PS50011; PROTEIN_KINASE_DOM
PS00108; PROTEIN_KINASE_ST