CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019527
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcriptional-regulating factor 1 
Protein Synonyms/Alias
 Breast cancer anti-estrogen resistance 2; Transcriptional-regulating protein 132; Zinc finger protein rapa; Zinc finger transcription factor TReP-132 
Gene Name
 TRERF1 
Gene Synonyms/Alias
 BCAR2; RAPA; TREP132 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
72LGLPVGSKNLGQMDTubiquitination[1]
188LRQLLSQKPMEPPAPubiquitination[1, 2]
812HKATLVWKPWPELENubiquitination[3, 4]
1154LDQLSLIKPIKDVDIacetylation[5]
Reference
 [1] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Activates transcription of CYP11A1. Interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1. 
Sequence Annotation
 DOMAIN 779 870 ELM2.
 DOMAIN 885 936 SANT.
 ZN_FING 512 534 C2H2-type 1.
 ZN_FING 1013 1037 C2H2-type 2.
 ZN_FING 1086 1108 C2H2-type 3.
 REGION 439 1200 Interacts with CREBBP.
 MOD_RES 767 767 Phosphothreonine.
 MOD_RES 954 954 Phosphothreonine.
 MOD_RES 955 955 Phosphoserine.
 MOD_RES 975 975 Phosphoserine (By similarity).  
Keyword
 Activator; Alternative splicing; Complete proteome; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1200 AA 
Protein Sequence
MGDQQLYKTN HVAHGSENLF YQQPPLGVHS GLNHNYGNAV TGGGMDAPQA SPISPHFPQD 60
TRDGLGLPVG SKNLGQMDTS RQGGWGSHAG PGNHVQLRGN LANSNMMWGA PAQAEPTDGY 120
QYTYSQASEI RTQKLTSGVL HKLDSFTQVF ANQNLRIQVN NMAQVLHTQS AVMDGAPDSA 180
LRQLLSQKPM EPPAPAIPSR YQQVPQQPHP GFTGGLSKPA LQVGQHPTQG HLYYDYQQPL 240
AQVPVQGGQP LQAPQMLSQH MQQMQQHQYY PPQQQQQAGQ QRISMQEIQT QPQQIRPSQP 300
QPPPQQQQPQ QLQLQQRQGS MQIPQYYQPQ PMMQHLQEQQ QQQMHLQPPS YHRDPHQYTP 360
EQAHTVQLIP LGSMSQYYYQ EPQQPYSHPL YQQSHLSQHQ QREDSQLKTY SSDRQAQAML 420
SSHGDLGPPD TGMGDPASSD LTRVSSTLPH RPLLSPSGIH LNNMGPQHQQ LSPSAMWPQM 480
HLPDGRAQPG SPESSGQPKG AFGEQFDAKN KLTCSICLKE FKNLPALNGH MRSHGGMRAS 540
PNLKQEEGEK VLPPQPQPPL PPPPPPPPPP QLPPEAESLT PMVMPVSVPV KLLPPKPSSQ 600
GFTNSTVAAP SARDKPASSM SDDEMPVLEI PRKHQPSVPK AEEPLKTVQE KKKFRHRPEP 660
LFIPPPPSYN PNPAASYSGA TLYQSQLRSP RVLGDHLLLD PTHELPPYTP PPMLSPVRQG 720
SGLFSNVLIS GHGPGAHPQL PLTPLTPTPR VLLCRSNSID GSNVTVTPGP GEQTVDVEPR 780
INIGLRFQAE IPELQDISAL AQDTHKATLV WKPWPELENH DLQQRVENLL NLCCSSALPG 840
GGTNSEFALH SLFEAKGDVM VALEMLLLRK PVRLKCHPLA NYHYAGSDKW TSLERKLFNK 900
ALATYSKDFI FVQKMVKSKT VAQCVEYYYT WKKIMRLGRK HRTRLAEIID DCVTSEEEEE 960
LEEEEEEDPE EDRKSTKEEE SEVPKSPEPP PVPVLAPTEG PPLQALGQPS GSFICEMPNC 1020
GAVFSSRQAL NGHARIHGGT NQVTKARGAI PSGKQKPGGT QSGYCSVKSS PSHSTTSGET 1080
DPTTIFPCKE CGKVFFKIKS RNAHMKTHRQ QEEQQRQKAQ KAAFAAEMAA TIERTTGPVG 1140
APGLLPLDQL SLIKPIKDVD ILDDDVVQQL GGVMEEAEVV DTDLLLDDQD SVLLQGDAEL 1200 
Gene Ontology
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IEA:InterPro.
 GO:0008301; F:DNA binding, bending; NAS:UniProtKB.
 GO:0030374; F:ligand-dependent nuclear receptor transcription coactivator activity; NAS:UniProtKB.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; NAS:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006707; P:cholesterol catabolic process; NAS:UniProtKB.
 GO:0042592; P:homeostatic process; NAS:UniProtKB.
 GO:0007275; P:multicellular organismal development; NAS:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0046885; P:regulation of hormone biosynthetic process; NAS:UniProtKB.
 GO:0006694; P:steroid biosynthetic process; NAS:UniProtKB. 
Interpro
 IPR000949; ELM2_dom.
 IPR009057; Homeodomain-like.
 IPR001005; SANT/Myb.
 IPR017884; SANT_dom.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like. 
Pfam
 PF01448; ELM2 
SMART
 SM00717; SANT
 SM00355; ZnF_C2H2 
PROSITE
 PS51156; ELM2
 PS51293; SANT
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS