CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020402
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Uveal autoantigen with coiled-coil domains and ankyrin repeats 
Protein Synonyms/Alias
  
Gene Name
 UACA 
Gene Synonyms/Alias
 KIAA1561 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
253LDILTLLKTASENTNubiquitination[1]
562GASAEVGKLRNQIKQacetylation[2]
730YLDNKLLKEQAHNLTubiquitination[3, 4]
Reference
 [1] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Regulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1 (By similarity). 
Sequence Annotation
 REPEAT 38 66 ANK 1.
 REPEAT 67 96 ANK 2.
 REPEAT 100 129 ANK 3.
 REPEAT 133 162 ANK 4.
 REPEAT 166 195 ANK 5.
 REPEAT 199 228 ANK 6.  
Keyword
 Alternative splicing; ANK repeat; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Nucleus; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1416 AA 
Protein Sequence
MKSLKSRLRR QDVPGPASSG AAAASAHAAD WNKYDDRLMK AAERGDVEKV TSILAKKGVN 60
PGKLDVEGRS VFHVVTSKGN LECLNAILIH GVDITTSDTA GRNALHLAAK YGHALCLQKL 120
LQYNCPTEHA DLQGRTALHD AAMADCPSSI QLLCDHGASV NAKDVDGRTP LVLATQMSRP 180
TICQLLIDRG ADVNSRDKQN RTALMLGCEY GCRDAVEVLI KNGADISLLD ALGHDSSYYA 240
RIGDNLDILT LLKTASENTN KGRELWKKGP SLQQRNLTHM QDEVNVKSHQ REHQNIQDLE 300
IENEDLKERL RKIQQEQRIL LDKVNGLQLQ LNEEVMVADD LESEREKLKS LLAAKEKQHE 360
ESLRTIEALK NRFKYFESDH LGSGSHFSNR KEDMLLKQGQ MYMADSQCTS PGIPAHMQSR 420
SMLRPLELSL PSQTSYSENE ILKKELEAMR TFCESAKQDR LKLQNELAHK VAECKALALE 480
CERVKEDSDE QIKQLEDALK DVQKRMYESE GKVKQMQTHF LALKEHLTSE AASGNHRLTE 540
ELKDQLKDLK VKYEGASAEV GKLRNQIKQN EMIVEEFKRD EGKLIEENKR LQKELSMCEM 600
EREKKGRKVT EMEGQAKELS AKLALSIPAE KFENMKSSLS NEVNEKAKKL VEMEREHEKS 660
LSEIRQLKRE LENVKAKLAQ HVKPEEHEQV KSRLEQKSGE LGKKITELTL KNQTLQKEIE 720
KVYLDNKLLK EQAHNLTIEM KNHYVPLKVS EDMKKSHDAI IDDLNRKLLD VTQKYTEKKL 780
EMEKLLLEND SLSKDVSRLE TVFVPPEKHE KEIIALKSNI VELKKQLSEL KKKCGEDQEK 840
IHALTSENTN LKKMMSNQYV PVKTHEEVKM TLNDTLAKTN RELLDVKKKF EDINQEFVKI 900
KDKNEILKRN LENTQNQIKA EYISLAEHEA KMSSLSQSMR KVQDSNAEIL ANYRKGQEEI 960
VTLHAEIKAQ KKELDTIQEC IKVKYAPIVS FEECERKFKA TEKELKDQLS EQTQKYSVSE 1020
EEVKKNKQEN DKLKKEIFTL QKDLRDKTVL IEKSHEMERA LSRKTDELNK QLKDLSQKYT 1080
EVKNVKEKLV EENAKQTSEI LAVQNLLQKQ HVPLEQVEAL KKSLNGTIEN LKEELKSMQR 1140
CYEKEQQTVT KLHQLLENQK NSSVPLAEHL QIKEAFEKEV GIIKASLREK EEESQNKMEE 1200
VSKLQSEVQN TKQALKKLET REVVDLSKYK ATKSDLETQI SSLNEKLANL NRKYEEVCEE 1260
VLHAKKKEIS AKDEKELLHF SIEQEIKDQK ERCDKSLTTI TELQRRIQES AKQIEAKDNK 1320
ITELLNDVER LKQALNGLSQ LTYTSGNPTK RQSQLIDTLQ HQVKSLEQQL ADADRQHQEV 1380
IAIYRTHLLS AAQGHMDEDV QEALLQIIQM RQGLVC 1416 
Gene Ontology
 GO:0043293; C:apoptosome; IEA:Compara.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005576; C:extracellular region; NAS:UniProtKB.
 GO:0016020; C:membrane; IEA:Compara.
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005635; C:nuclear envelope; IEA:Compara.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0008630; P:intrinsic apoptotic signaling pathway in response to DNA damage; IEA:Compara.
 GO:0008631; P:intrinsic apoptotic signaling pathway in response to oxidative stress; IEA:Compara.
 GO:0050728; P:negative regulation of inflammatory response; IEA:Compara.
 GO:0042347; P:negative regulation of NF-kappaB import into nucleus; IEA:Compara.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0043280; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Compara.
 GO:0042307; P:positive regulation of protein import into nucleus; IEA:Compara.
 GO:0009411; P:response to UV; IEA:Compara. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR000727; T_SNARE_dom. 
Pfam
 PF12796; Ank_2 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50192; T_SNARE 
PRINTS
 PR01415; ANKYRIN.