CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042489
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Mediator of RNA polymerase II transcription subunit 12 
Protein Synonyms/Alias
  
Gene Name
 MED12 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
28GDEHGSAKNVSFNPAubiquitination[1]
36NVSFNPAKISSNFSSubiquitination[1, 2]
48FSSIIAEKLRCNTLPacetylation[3]
48FSSIIAEKLRCNTLPubiquitination[1, 4, 5]
92FTDLAGTKPLTQLAKubiquitination[1]
100PLTQLAKKVPIFSKKubiquitination[1]
106KKVPIFSKKEEVFGYubiquitination[1]
107KVPIFSKKEEVFGYLubiquitination[1]
397REIEQQIKERGQAVEubiquitination[1, 2]
411EVRWSFDKCQEATAGubiquitination[1, 2, 5]
495HRAMVVAKLLEKRQAubiquitination[1, 2]
499VVAKLLEKRQAEIEAubiquitination[1]
730VVLFGVGKQRDDARHubiquitination[1, 2]
748KITKDILKVLNRKGTubiquitination[1, 4]
753ILKVLNRKGTAETDQubiquitination[1, 2]
782LGGEDGQKRRRNRPEubiquitination[1, 2, 5]
800TAEDIFAKFQHLSHYubiquitination[1]
956SCSHLKNKFGELFSDubiquitination[1]
967LFSDFCSKVKNTIYCubiquitination[1, 6]
969SDFCSKVKNTIYCNVubiquitination[1, 2]
1149RILLHLFKTPQLNPCubiquitination[1]
1162PCQSDGNKPTVGIRSubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2127 AA 
Protein Sequence
EDELTALNVK QGFNNQPAVS GDEHGSAKNV SFNPAKISSN FSSIIAEKLR CNTLPDTGRR 60
KPQVNQKDNF WLVTARSQSA INTWFTDLAG TKPLTQLAKK VPIFSKKEEV FGYLAKYTVP 120
VMRAAWLIKM TCAYYAAISE TKVKKRHVDP FMEWTQIITK YLWEQLQKMA EYYRPGPAGS 180
GGCGSTIGPL PHDVEVAIRQ WDYTEKLAMF MFQDGMLDRH EFLTWVLECF EKIRPGEDEL 240
LKLLLPLLLR YSGEFVQSAY LSRRLAYFCT RRLALQLDGV SSHSSHVISA QSTSTLPTTP 300
APQPPTSSTP STPFSDLLMC PQHRPLVFGL SCILQTILLC CPSALVWHYS LTDSRIKTGS 360
PLDHLPIAPS NLPMPEGNSA FTQQVRAKLR EIEQQIKERG QAVEVRWSFD KCQEATAGFT 420
IGRVLHTLEV LDSHSFERSD FSNSLDSLCN RIFGLGPSKD GHEISSDDDA VVSLLCEWAV 480
SCKRSGRHRA MVVAKLLEKR QAEIEAERCG ESEAADEKGS IASGSLSAPS APIFQDVLLQ 540
FLDTQAPMLT DPRSESERVE FFNLVLLFCE LIRHDVFSHN MYTCTLISRG DLAFGAPGPR 600
PPSPFDDPAD DPEHKEAEGS SSSKLEDPGL SESMDIDPSS SVLFEDMEKP DFSLFSPTMP 660
CEGKGSPSPE KPDVEKEVKP PPKEKIEGTL GVLYDQPRHV QYATHFPIPQ EESCSHECNQ 720
RLVVLFGVGK QRDDARHAIK KITKDILKVL NRKGTAETDQ LAPIVPLNPG DLTFLGGEDG 780
QKRRRNRPEA FPTAEDIFAK FQHLSHYDQH QVTAQVSRNV LEQITSFALG MSYHLPLVQH 840
VQFIFDLMEY SLSISGLIDF AIQLLNELSV VEAELLLKSS DLVGSYTTSL CLCIVAVLRH 900
YHACLILNQD QMAQVFEGLC GVVKHGMNRS DGSSAERCIL AYLYDLYTSC SHLKNKFGEL 960
FSDFCSKVKN TIYCNVEPSE SNMRWAPEFM IDTLENPAAH TFTYTGLGKS LSENPANRYS 1020
FVCNALMHVC VGHHDPDRVN DIAILCAELT GYCKSLSAEW LGVLKALCCS SNNGTCGFND 1080
LLCNVDVSDL SFHDSLATFV AILIARQCLL LEDLIRCAAI PSLLNAACSE QDSEPGARLT 1140
CRILLHLFKT PQLNPCQSDG NKPTVGIRSS CDRHLLAASQ NRIVDGAVFA VLKAVFVLGD 1200
AELKGSGFTV TGGTEELPEE EGGGGSGGRR QGGRNISVET ASLDVYAKYV LRSICQQEWV 1260
GERCLKSLCE DSNDLQDPVL SSAQAQRLMQ LICYPHRLLD NEDGENPQRQ RIKRILQNLD 1320
QWTMRQSSLE LQLMIKQTPN NEMNSLLENI AKATIEVFQQ SAETGSSSGS TASNMPSSSK 1380
TKPVLSSLER SGVWLVAPLI AKLPTSVQGH VLKAAGEELE KGQHLGSSSR KERDRQKQKS 1440
MSLLSQQPFL SLVLTCLKGQ DEQREGLLTS LYSQVHQIVN NWRDDQYLDD CKPKQLMHEA 1500
LKLRLNLVGG MFDTVQRSTQ QTTEWAMLLL EIIISGTVDM QSNNELFTTV LDMLSVLING 1560
TLAADMSSIS QGSMEENKRA YMNLAKKLQK ELGERQSDSL EKVRQLLPLP KQTRDVITCE 1620
PQGSLIDTKG NKIAGFDSIF KKEGLQVSTK QKISPWDLFE GLKPSAPLSW GWFGTVRVDR 1680
RVARGEEQQR LLLYHTHLRP RPRAYYLEPL PLPPEDEEPP APTLLEPEKK APEPPKTDKP 1740
GAAPPSTEER KKKSTKGKKR SQPATKTEDY GMGPGRSGPY GVTVPPDLLH HPNPGSITHL 1800
NYRQGSIGLY TQNQPLPAGG PRVDPYRPVR LPMQKLPTRP TYPGVLPTTM TGVMGLEPSS 1860
YKTSVYRQQQ PAVPQGQRLR QQLQQSQGML GQSSVHQMTP SSSYGLQTSQ LSPSSLQGYT 1920
PYVSHVGLQQ HTGPADPTRH LQQRPSGYVH QQAPTYGHGL TSTQRFSHQT LQQTPMISTM 1980
TPMSAQGVQA GVRSTAILPE QQQQQQQQQQ QQQQQQQQQQ QQQQQQYHIR QQQQQQILRQ 2040
QQQQQQQQQQ QQQQQQQQQQ QQQQQHQQQQ QQQAAPPQPQ PQSQPQFQRQ GLQQTQQQQQ 2100
TAALVRQLQQ QLSNTQPQPS TNIFGRY 2127 
Gene Ontology
 GO:0016592; C:mediator complex; IEA:InterPro.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IEA:InterPro. 
Interpro
 IPR019035; Mediator_Med12.
 IPR021989; Mediator_Med12_catenin-bd.
 IPR021990; Mediator_Med12_LCEWAV. 
Pfam
 PF09497; Med12
 PF12145; Med12-LCEWAV
 PF12144; Med12-PQL 
SMART
  
PROSITE
  
PRINTS