CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021821
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 24-hydroxycholesterol 7-alpha-hydroxylase 
Protein Synonyms/Alias
 Cytochrome P450 39A1; mCYP39A1; Oxysterol 7-alpha-hydroxylase 
Gene Name
 Cyp39a1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
179ATLNTLFKKGLFLTDubiquitination[1]
187KGLFLTDKRTIKEFYubiquitination[1]
191LTDKRTIKEFYQQFKubiquitination[1]
313SVFGTAGKDKIKVSEubiquitination[1]
387FPEPESFKPERWKEAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Involved in the bile acid metabolism. Has a preference for 24-hydroxycholesterol, and converts it into a 7-alpha- hydroxylated product (By similarity). 
Sequence Annotation
 METAL 415 415 Iron (heme axial ligand) (Potential).  
Keyword
 Bile acid catabolism; Complete proteome; Endoplasmic reticulum; Heme; Iron; Lipid degradation; Lipid metabolism; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Reference proteome; Steroid metabolism. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 470 AA 
Protein Sequence
MGIMELFSPI AIAVLGSCVL FLFSRLKNLL GPPCIQGWIP WIGAGLEFGK APLEFIEKAR 60
IKYGPVFTIF AMGNRMTFVS EEEGINVLLK SEHVDFESAV QSPVYHTAWI PKNVFSALHE 120
RLYALMKGKM GTFNTHHFTG PLTEELHEQL EGLGTHGTMD LNDFVRYLLY PATLNTLFKK 180
GLFLTDKRTI KEFYQQFKTY DEGFEYGSQL PEWLLRNWSK SKRWLLALFE KNIGNIKAHG 240
SAGHSGTLLQ AILEVVETET RQYSPNYGLV VLWAALANAP PIAFWTLGYI LSHPDIHRTV 300
LESISSVFGT AGKDKIKVSE DDLKKLLIIK WCILESVRLR APGVITRKVV KPVKILNHTV 360
PSGDLLMLSP FWLHRNPKYF PEPESFKPER WKEANLDKYI FLDYFMAFGG GKFQCPGRWF 420
ALLEIQLCII LVLYKYECSL LDPLPKQSSR HLVGVPQPAG KCRIEYKQRA 470 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0043231; C:intracellular membrane-bounded organelle; TAS:UniProtKB.
 GO:0033782; F:24-hydroxycholesterol 7alpha-hydroxylase activity; IEA:EC.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0008396; F:oxysterol 7-alpha-hydroxylase activity; IDA:UniProtKB.
 GO:0008387; F:steroid 7-alpha-hydroxylase activity; IDA:MGI.
 GO:0006699; P:bile acid biosynthetic process; IDA:UniProtKB.
 GO:0030573; P:bile acid catabolic process; IEA:UniProtKB-KW.
 GO:0006707; P:cholesterol catabolic process; IDA:MGI.
 GO:0007586; P:digestion; TAS:UniProtKB. 
Interpro
 IPR001128; Cyt_P450.
 IPR024204; Cyt_P450_CYP7A1-type.
 IPR002403; Cyt_P450_E_grp-IV. 
Pfam
 PF00067; p450 
SMART
  
PROSITE
 PS00086; CYTOCHROME_P450 
PRINTS
 PR00465; EP450IV.