CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023123
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Guanine nucleotide exchange factor VAV3 
Protein Synonyms/Alias
 VAV-3 
Gene Name
 VAV3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
201SCCLAEIKQTEEKYTubiquitination[1]
311LKLEECSKRANNGKFubiquitination[1]
317SKRANNGKFTLRDLLubiquitination[1]
528FTRVTSCKVCQMLLRubiquitination[1]
574SGEQGTLKLPEKRTNubiquitination[1]
588NGLRRTPKQVDPGLPubiquitination[1]
752YYKHHSLKEGFRTLDubiquitination[1]
767TTLQFPYKEPEHSAGubiquitination[1]
788GNSLLSPKVLGIAIAubiquitination[1]
811MRELSLLKGDVVKIYubiquitination[1]
820DVVKIYTKMSANGWWubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1- induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)- mediated adhesion. Does not affect integrin beta-1 (ITGB1)- mediated adhesion (By similarity). 
Sequence Annotation
 DOMAIN 1 119 CH.
 DOMAIN 192 371 DH.
 DOMAIN 400 502 PH.
 DOMAIN 592 660 SH3 1.
 DOMAIN 672 766 SH2.
 DOMAIN 788 847 SH3 2.
 ZN_FING 513 562 Phorbol-ester/DAG-type.
 REGION 560 847 Sufficient for interaction with ROS1.
 MOD_RES 141 141 Phosphotyrosine.  
Keyword
 3D-structure; Alternative promoter usage; Alternative splicing; Angiogenesis; Complete proteome; Guanine-nucleotide releasing factor; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; SH2 domain; SH3 domain; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 847 AA 
Protein Sequence
MEPWKQCAQW LIHCKVLPTN HRVTWDSAQV FDLAQTLRDG VLLCQLLNNL RAHSINLKEI 60
NLRPQMSQFL CLKNIRTFLT ACCETFGMRK SELFEAFDLF DVRDFGKVIE TLSRLSRTPI 120
ALATGIRPFP TEESINDEDI YKGLPDLIDE TLVEDEEDLY DCVYGEDEGG EVYEDLMKAE 180
EAHQPKCPEN DIRSCCLAEI KQTEEKYTET LESIEKYFMA PLKRFLTAAE FDSVFINIPE 240
LVKLHRNLMQ EIHDSIVNKN DQNLYQVFIN YKERLVIYGQ YCSGVESAIS SLDYISKTKE 300
DVKLKLEECS KRANNGKFTL RDLLVVPMQR VLKYHLLLQE LVKHTTDPTE KANLKLALDA 360
MKDLAQYVNE VKRDNETLRE IKQFQLSIEN LNQPVLLFGR PQGDGEIRIT TLDKHTKQER 420
HIFLFDLAVI VCKRKGDNYE MKEIIDLQQY KIANNPTTDK ENKKWSYGFY LIHTQGQNGL 480
EFYCKTKDLK KKWLEQFEMA LSNIRPDYAD SNFHDFKMHT FTRVTSCKVC QMLLRGTFYQ 540
GYLCFKCGAR AHKECLGRVD NCGRVNSGEQ GTLKLPEKRT NGLRRTPKQV DPGLPKMQVI 600
RNYSGTPPPA LHEGPPLQLQ AGDTVELLKG DAHSLFWQGR NLASGEVGFF PSDAVKPCPC 660
VPKPVDYSCQ PWYAGAMERL QAETELINRV NSTYLVRHRT KESGEYAISI KYNNEAKHIK 720
ILTRDGFFHI AENRKFKSLM ELVEYYKHHS LKEGFRTLDT TLQFPYKEPE HSAGQRGNRA 780
GNSLLSPKVL GIAIARYDFC ARDMRELSLL KGDVVKIYTK MSANGWWRGE VNGRVGWFPS 840
TYVEEDE 847 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0005096; F:GTPase activator activity; TAS:ProtInc.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; EXP:Reactome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0030676; F:Rac guanyl-nucleotide exchange factor activity; IEA:Compara.
 GO:0005070; F:SH3/SH2 adaptor activity; TAS:ProtInc.
 GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0050853; P:B cell receptor signaling pathway; IMP:UniProtKB.
 GO:0016477; P:cell migration; IEA:Compara.
 GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0007229; P:integrin-mediated signaling pathway; IEA:Compara.
 GO:0030032; P:lamellipodium assembly; IEA:Compara.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0030890; P:positive regulation of B cell proliferation; IMP:UniProtKB.
 GO:0045785; P:positive regulation of cell adhesion; IEA:Compara.
 GO:0043552; P:positive regulation of phosphatidylinositol 3-kinase activity; IEA:Compara.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0006974; P:response to DNA damage stimulus; IMP:UniProtKB.
 GO:0042493; P:response to drug; IMP:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome.
 GO:0006906; P:vesicle fusion; IEA:Compara. 
Interpro
 IPR001715; CH-domain.
 IPR000219; DH-domain.
 IPR001331; GDS_CDC24_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000980; SH2.
 IPR011511; SH3_2.
 IPR001452; SH3_domain.
 IPR003096; SM22_calponin. 
Pfam
 PF00130; C1_1
 PF00307; CH
 PF00169; PH
 PF00621; RhoGEF
 PF00017; SH2
 PF07653; SH3_2 
SMART
 SM00109; C1
 SM00033; CH
 SM00233; PH
 SM00325; RhoGEF
 SM00252; SH2
 SM00326; SH3 
PROSITE
 PS50021; CH
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN
 PS50001; SH2
 PS50002; SH3
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS
 PR00401; SH2DOMAIN.
 PR00452; SH3DOMAIN.
 PR00888; SM22CALPONIN.