CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023238
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Melanoma-associated antigen D2 
Protein Synonyms/Alias
 11B6; Breast cancer-associated gene 1 protein; BCG-1; Hepatocellular carcinoma-associated protein JCL-1; MAGE-D2 antigen 
Gene Name
 MAGED2 
Gene Synonyms/Alias
 BCG1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
137ADTKVNTKAQETEAAubiquitination[1]
207TGGRRVSKALMASMAubiquitination[2, 3, 4, 5]
261KARRRAAKLQSSQEPubiquitination[1]
288GRANDLVKYLLAKDQubiquitination[1, 2, 3, 5]
293LVKYLLAKDQTKIPIubiquitination[3, 4]
297LLAKDQTKIPIKRSDubiquitination[4]
330RAGYSLEKVFGIQLKubiquitination[1, 2, 3, 4, 5]
397VIWEVLRKLGLRPGIubiquitination[2, 3, 5]
414SLFGDVKKLITDEFVubiquitination[3]
424TDEFVKQKYLDYARVubiquitination[3, 4]
453RSYYETSKMKVLKFAubiquitination[1, 4]
469KVQKKDPKEWAAQYRubiquitination[1, 2, 3, 4, 5]
484EAMEADLKAAAEAAAubiquitination[1, 4, 6]
494AEAAAEAKARAEIRAubiquitination[1, 4, 7]
528ADIGPWAKARIQAGAubiquitination[4, 8]
538IQAGAEAKAKAQESGubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
 DOMAIN 279 478 MAGE.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 5 5 Phosphoserine.
 MOD_RES 72 72 Phosphothreonine.
 MOD_RES 157 157 Phosphoserine.
 MOD_RES 190 190 Phosphoserine.
 MOD_RES 191 191 Phosphoserine.
 MOD_RES 194 194 Phosphoserine.
 MOD_RES 197 197 Phosphoserine.
 MOD_RES 244 244 Phosphoserine.
 MOD_RES 247 247 Phosphoserine.
 MOD_RES 265 265 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Phosphoprotein; Polymorphism; Reference proteome; Tumor antigen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 606 AA 
Protein Sequence
MSDTSESGAG LTRFQAEASE KDSSSMMQTL LTVTQNVEVP ETPKASKALE VSEDVKVSKA 60
SGVSKATEVS KTPEAREAPA TQASSTTQLT DTQVLAAENK SLAADTKKQN ADPQAVTMPA 120
TETKKVSHVA DTKVNTKAQE TEAAPSQAPA DEPEPESAAA QSQENQDTRP KVKAKKARKV 180
KHLDGEEDGS SDQSQASGTT GGRRVSKALM ASMARRASRG PIAFWARRAS RTRLAAWARR 240
ALLSLRSPKA RRGKARRRAA KLQSSQEPEA PPPRDVALLQ GRANDLVKYL LAKDQTKIPI 300
KRSDMLKDII KEYTDVYPEI IERAGYSLEK VFGIQLKEID KNDHLYILLS TLEPTDAGIL 360
GTTKDSPKLG LLMVLLSIIF MNGNRSSEAV IWEVLRKLGL RPGIHHSLFG DVKKLITDEF 420
VKQKYLDYAR VPNSNPPEYE FFWGLRSYYE TSKMKVLKFA CKVQKKDPKE WAAQYREAME 480
ADLKAAAEAA AEAKARAEIR ARMGIGLGSE NAAGPCNWDE ADIGPWAKAR IQAGAEAKAK 540
AQESGSASTG ASTSTNNSAS ASASTSGGFS AGASLTATLT FGLFAGLGGA GASTSGSSGA 600
CGFSYK 606 
Gene Ontology
  
Interpro
 IPR002190; MAGE. 
Pfam
 PF01454; MAGE 
SMART
  
PROSITE
 PS50838; MAGE 
PRINTS