CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001654
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TBC1 domain family member 8 
Protein Synonyms/Alias
 AD 3; Vascular Rab-GAP/TBC-containing protein 
Gene Name
 TBC1D8 
Gene Synonyms/Alias
 VRP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
119SFVKGKVKALIAEETubiquitination[1]
275QEPSQITKRDLEARAubiquitination[1, 2]
799QGHEDTTKQNVLRVVubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 May act as a GTPase-activating protein for Rab family protein(s). 
Sequence Annotation
 DOMAIN 145 212 GRAM 1.
 DOMAIN 285 353 GRAM 2.
 DOMAIN 505 692 Rab-GAP TBC.  
Keyword
 Alternative splicing; Complete proteome; GTPase activation; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1140 AA 
Protein Sequence
MWLKPEEVLL KNALKLWVTQ KSSCYFILQR RRGHGEGGGR LTGRLVGALD AVLDSNARVA 60
PFRILLQVPG SQVYSPIACG ATLEEINQHW DWLEQNLLHT LSVFDNKDDI ASFVKGKVKA 120
LIAEETSSRL AEQEEEPEKF REALVKFEAR FNFPEAEKLV TYYSCCCWKG RVPRQGWLYL 180
SINHLCFYSF FLGKELKLVV PWVDIQKLER TSNVFLTDTI RITTQNKERD FSMFLNLDEV 240
FKVMEQLADV TLRRLLDNEV FDLDPDLQEP SQITKRDLEA RAQNEFFRAF FRLPRKEKLH 300
AVVDCSLWTP FSRCHTTGRM FASDSYICFA SREDGCCKII LPLREVVSIE KMEDTSLLPH 360
PIIVSIRSKV AFQFIELRDR DSLVEALLAR LKQVHANHPV HYDTSADDDM ASLVFHSTSM 420
CSDHRFGDLE MMSSQNSEES EKEKSPLMHP DALVTAFQQS GSQSPDSRMS REQIKISLWN 480
DHFVEYGRTV CMFRTEKIRK LVAMGIPESL RGRLWLLFSD AVTDLASHPG YYGNLVEESL 540
GKCCLVTEEI ERDLHRSLPE HPAFQNETGI AALRRVLTAY AHRNPKIGYC QSMNILTSVL 600
LLYTKEEEAF WLLVAVCERM LPDYFNHRVI GAQVDQSVFE ELIKGHLPEL AEHMNDLSAL 660
ASVSLSWFLT LFLSIMPLES AVNVVDCFFY DGIKAIFQLG LAVLEANAED LCSSKDDGQA 720
LMILSRFLDH IKNEDSPGPP VGSHHAFFSD DQEPYPVTDI SDLIRDSYEK FGDQSVEQIE 780
HLRYKHRIRV LQGHEDTTKQ NVLRVVIPEV SILPEDLEEL YDLFKREHMM SCYWEQPRPM 840
ASRHDPSRPY AEQYRIDARQ FAHLFQLVSP WTCGAHTEIL AERTFRLLDD NMDQLIEFKA 900
FVSCLDIMYN GEMNEKIKLL YRLHIPPALT ENDRDSQSPL RNPLLSTSRP LVFGKPNGDA 960
VDYQKQLKQM IKDLAKEKDK TEKELPKMSQ REFIQFCKTL YSMFHEDPEE NDLYQAIATV 1020
TTLLLQIGEV GQRGSSSGSC SQECGEELRA SAPSPEDSVF ADTGKTPQDS QAFPEAAERD 1080
WTVSLEHILA SLLTEQSLVN FFEKPLDMKS KLENAKINQY NLKTFEMSHQ SQSELKLSNL 1140 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0016020; C:membrane; TAS:ProtInc.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005097; F:Rab GTPase activator activity; IEA:InterPro.
 GO:0008015; P:blood circulation; TAS:ProtInc.
 GO:0008284; P:positive regulation of cell proliferation; TAS:ProtInc.
 GO:0032851; P:positive regulation of Rab GTPase activity; IEA:GOC. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR004182; GRAM.
 IPR000195; Rab-GTPase-TBC_dom. 
Pfam
 PF02893; GRAM
 PF00566; RabGAP-TBC 
SMART
 SM00568; GRAM
 SM00164; TBC 
PROSITE
 PS50086; TBC_RABGAP 
PRINTS