UniProt Accession

 DCNL1_HUMAN; Q96GG9; B2RB37; Q7L3G9; Q8TEX7; Q9H6M1; Q9HCT3 

Genbank Protein ID

 AF292100; AF456425; AF456426; AK025764; AK056335; AK314480; CH471052; BC009478; BC013163 

Genbank Nucleotide ID

 AAG00606.2; AAL78672.1; AAL78673.1; BAB15235.1; BAG51680.1; BAG37084.1; EAW78342.1; AAH09478.1; AAH13163.2 

Protein Name

 DCN1-like protein 1 

Protein Synonyms/Alias

 DCUN1 domain-containing protein 1; Defective in cullin neddylation protein 1-like protein 1; Squamous cell carcinoma-related oncogene 

Gene Name

 DCUN1D1 

Gene Synonyms/Alias

 DCUN1L1; RP42; SCCRO 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
57	LYIRESVKGSLDRKK	ubiquitination	[1, 2, 3]
63	VKGSLDRKKLEQLYN	ubiquitination	[2, 3, 4, 5, 6, 7, 8]
64	KGSLDRKKLEQLYNR	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8]
80	KDPQDENKIGIDGIQ	ubiquitination	[3]
138	CDSIEKLKAQIPKME	ubiquitination	[2]
143	KLKAQIPKMEQELKE	ubiquitination	[1, 2, 3, 4, 5, 7, 8]
149	PKMEQELKEPGRFKD	ubiquitination	[1, 2, 3, 5, 6, 7, 8, 9]
155	LKEPGRFKDFYQFTF	ubiquitination	[7]
191	LVLNGRFKFLDLWNK	ubiquitination	[5, 8]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Part of an E3 ubiquitin ligase complex for neddylation. Required for neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes by enhancing the rate of cullins neddylation. Functions to recruit the NEDD8-charged E2 enzyme to the cullin component. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression (By similarity). 

Sequence Annotation

 DOMAIN 8 45 UBA-like.
 DOMAIN 60 248 DCUN1.  

Keyword

 3D-structure; Complete proteome; Proto-oncogene; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 259 AA 

Protein Sequence

Gene Ontology

 GO:0000151; C:ubiquitin ligase complex; IDA:UniProtKB. 

Interpro

 IPR014764; DCN-prot.
 IPR005176; PONY_dom.
 IPR009060; UBA-like. 

Pfam

 PF03556; Cullin_binding 

SMART

  

PROSITE

 PS51229; DCUN1 

PRINTS