CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000795
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cyclic AMP-responsive element-binding protein 3 
Protein Synonyms/Alias
 CREB-3; cAMP-responsive element-binding protein 3; Leucine zipper protein; Luman; Transcription factor LZIP-alpha; Processed cyclic AMP-responsive element-binding protein 3; N-terminal Luman; Transcriptionally active form 
Gene Name
 CREB3 
Gene Synonyms/Alias
 LZIP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
156EEKSLLEKEGLILPEubiquitination[1, 2, 3]
208GLESRVLKYTAQNMEubiquitination[1, 2, 3]
376LQANLTRKGGWLPTGubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Endoplasmic reticulum (ER)-bound transcription factor that plays a role in the unfolded protein response (UPR). Involved in cell proliferation and migration, tumor suppression and inflammatory gene expression. Plays also a role in the human immunodeficiency virus type 1 (HIV-1) virus protein expression and in the herpes simplex virus-1 (HSV-1) latent infection and reactivation from latency. Isoform 2 plays a role in the unfolded protein response (UPR). Isoform 2 acts as a positive regulator of LKN-1/CCL15-induced chemotaxis signaling of leukocyte cell migration. Isoform 2 may play a role as a cellular tumor suppressor that is targeted by the hepatitis C virus (HSV) core protein. Isoform 2 represses the VP16-mediated transactivation of immediate early genes of the HSV-1 virus by sequestring host cell factor-1 HCFC1 in the ER membrane of sensory neurons, thereby preventing the initiation of the replicative cascade leading to latent infection. Isoform 3 functions as a negative transcriptional regulator in ligand-induced transcriptional activation of the glucocorticoid receptor NR3C1 by recruiting and activating histone deacetylases (HDAC1, HDAC2 and HDAC6). Isoform 3 decreases the acetylation level of histone H4. Isoform 3 does not promote the chemotactic activity of leukocyte cells. 
Sequence Annotation
 DOMAIN 174 237 bZIP.
 REGION 1 92 Transcription activation (acidic).
 REGION 176 208 Basic motif (By similarity).
 REGION 216 237 Leucine-zipper (By similarity).
 MOTIF 13 17 LXXLL motif 1.
 MOTIF 54 58 LXXLL motif 2.
 MOTIF 78 81 HCFC1-binding-motif (HBM).
 CARBOHYD 331 331 N-linked (GlcNAc...) (Potential).
 CARBOHYD 372 372 N-linked (GlcNAc...) (Potential).  
Keyword
 Activator; Alternative splicing; Chemotaxis; Complete proteome; Cytoplasm; DNA-binding; Endoplasmic reticulum; Glycoprotein; Host-virus interaction; Membrane; Nucleus; Reference proteome; Repeat; Repressor; Signal-anchor; Transcription; Transcription regulation; Transmembrane; Transmembrane helix; Unfolded protein response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 395 AA 
Protein Sequence
MELELDAGDQ DLLAFLLEES GDLGTAPDEA VRAPLDWALP LSEVPSDWEV DDLLCSLLSP 60
PASLNILSSS NPCLVHHDHT YSLPRETVSM DLGECEISLT GRTGFMGLAI HTFPFAESES 120
CRKEGTQMTP QHMEELAEQE IARLVLTDEE KSLLEKEGLI LPETLPLTKT EEQILKRVRR 180
KIRNKRSAQE SRRKKKVYVG GLESRVLKYT AQNMELQNKV QLLEEQNLSL LDQLRKLQAM 240
VIEISNKTSS SSTCILVLLV SFCLLLVPAM YSSDTRGSLP AEHGVLSRQL RALPSEDPYQ 300
LELPALQSEV PKDSTHQWLD GSDCVLQAPG NTSCLLHYMP QAPSAEPPLE WPFPDLFSEP 360
LCRGPILPLQ ANLTRKGGWL PTGSPSVILQ DRYSG 395 
Gene Ontology
 GO:0005829; C:cytosol; IMP:UniProtKB.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; IDA:UniProtKB.
 GO:0030176; C:integral to endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0043025; C:neuronal cell body; IDA:UniProtKB.
 GO:0016604; C:nuclear body; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0008140; F:cAMP response element binding protein binding; IDA:UniProtKB.
 GO:0031726; F:CCR1 chemokine receptor binding; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IDA:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0006935; P:chemotaxis; IEA:UniProtKB-KW.
 GO:0042994; P:cytoplasmic sequestering of transcription factor; IDA:UniProtKB.
 GO:0019043; P:establishment of viral latency; IDA:UniProtKB.
 GO:0050930; P:induction of positive chemotaxis; IDA:UniProtKB.
 GO:0045786; P:negative regulation of cell cycle; NAS:UniProtKB.
 GO:2000326; P:negative regulation of ligand-dependent nuclear receptor transcription coactivator activity; IDA:UniProtKB.
 GO:0051928; P:positive regulation of calcium ion transport; IMP:UniProtKB.
 GO:0030335; P:positive regulation of cell migration; IMP:UniProtKB.
 GO:0090045; P:positive regulation of deacetylase activity; IDA:UniProtKB.
 GO:0002230; P:positive regulation of defense response to virus by host; IDA:UniProtKB.
 GO:0090026; P:positive regulation of monocyte chemotaxis; IDA:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0006990; P:positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response; IDA:UniProtKB.
 GO:0001558; P:regulation of cell growth; IDA:UniProtKB.
 GO:0042127; P:regulation of cell proliferation; IMP:UniProtKB.
 GO:0019046; P:release from viral latency; IDA:UniProtKB.
 GO:0006986; P:response to unfolded protein; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IDA:UniProtKB.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR004827; bZIP.
 IPR008917; Euk_TF_DNA-bd. 
Pfam
 PF00170; bZIP_1 
SMART
 SM00338; BRLZ 
PROSITE
 PS50217; BZIP
 PS00036; BZIP_BASIC 
PRINTS