| Tag | Content |
|---|
| CPLM ID | CPLM-011757 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Transcriptional coactivator HFI1/ADA1 |
Protein Synonyms/Alias | |
Gene Name | HFI1 |
Gene Synonyms/Alias | ADA1; SUP110; YPL254W |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 234 | VTNPESLKRVKSNNL | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. HFI1/ADA1 and SPT20/ADA5 may recruit TATA binding protein (TBP) and possibly other basal factors to bind to the TATA box. |
Sequence Annotation | |
Keyword | Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 488 AA |
Protein Sequence | MSAIQSPAPK PLQPTYPAAS PASTNAYMKP GLIGSPAVSN HTEPNNGNNE TAEPQGPNQR 60
IDLGAMIEEL TSLLGKESWT KYAQIISLFI LGKLSRKELS NELELVFSPS AASLEKSNTN 120
HHHSLVRLHN QLLLGIFANS LRENPLGRNG NESSWGFGNG SNNPNNKLKR INKHNSQIEV 180
YKKIVMSLPL NDRNRLKMIT KEAGKRGFIF CSVFQARLNN IPKIPIVTNP ESLKRVKSNN 240
LKTPLEWSQD IMNGFNVPLA SESHSLPDTD SFYLRMVGIA REHGLVGTVD ARCVELISLA 300
LDQYLKNIIE FTIDTVRYRR KKYSDYYDLN ESGLYKSVSE MAADKRDAKI KQLDDDKNED 360
ECADEAKSIN NGNNSSKDDI GDISMSSITK AGEAVNEELH ENRTISLTNE DIYDSLSIFP 420
NLVEPSGSYY ALTNLGLVND DELVDMKSNI DDLPDFLNEK PTFTPLDERN VGTRHELNWL 480
IKGILTED 488 |
Gene Ontology | GO:0005671; C:Ada2/Gcn5/Ada3 transcription activator complex; IDA:SGD. GO:0000124; C:SAGA complex; IDA:SGD. GO:0046695; C:SLIK (SAGA-like) complex; IDA:SGD. GO:0003713; F:transcription coactivator activity; IMP:SGD. GO:0016573; P:histone acetylation; IDA:SGD. GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW. GO:0006366; P:transcription from RNA polymerase II promoter; IMP:SGD. |
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