UniProt Accession

 RMLB1_ECOLI; P37759; P78082 

Genbank Protein ID

 U09876; U00096; AP009048 

Genbank Nucleotide ID

 AAB88398.1; AAC75102.1; BAA15883.1 

Protein Name

 dTDP-glucose 4,6-dehydratase 1 

Protein Synonyms/Alias

  

Gene Name

 rfbB 

Gene Synonyms/Alias

 rmlB; b2041; JW2026 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
33	DSVVNVDKLTYAGNR	acetylation	[1]
122	WSALDSDKKNSFRFH	acetylation	[1]
226	KALPIYGKGDQIRDW	acetylation	[1]
251	YTVVTEGKAGETYNI	acetylation	[1]
310	RYAIDAEKIGRALGW	acetylation	[1]
318	IGRALGWKPQETFES	acetylation	[1]
339	EWYLSNTKWVDNVKS	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction (By similarity). 

Sequence Annotation

 NP_BIND 7 13 NAD (Potential).
 NP_BIND 32 35 NAD (By similarity).
 NP_BIND 58 59 NAD (By similarity).
 NP_BIND 167 171 NAD (By similarity).
 REGION 133 135 Substrate binding (By similarity).
 REGION 206 207 Substrate binding (By similarity).
 REGION 222 224 Substrate binding (By similarity).
 REGION 297 300 Substrate binding (By similarity).
 ACT_SITE 134 134 Proton donor (By similarity).
 ACT_SITE 135 135 Proton acceptor (By similarity).
 ACT_SITE 167 167 Proton acceptor (By similarity).
 BINDING 80 80 NAD; via carbonyl oxygen (By similarity).
 BINDING 84 84 Substrate; via carbonyl oxygen (By
 BINDING 99 99 NAD (By similarity).
 BINDING 196 196 Substrate (By similarity).
 BINDING 197 197 NAD; via amide nitrogen (By similarity).
 BINDING 231 231 Substrate (By similarity).
 BINDING 266 266 Substrate (By similarity).
 BINDING 357 357 Substrate (By similarity).  

Keyword

 Complete proteome; Lipopolysaccharide biosynthesis; Lyase; NAD; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 361 AA 

Protein Sequence

Gene Ontology

 GO:0050662; F:coenzyme binding; IEA:InterPro.
 GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; ISS:UniProtKB.
 GO:0019305; P:dTDP-rhamnose biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0045226; P:extracellular polysaccharide biosynthetic process; ISS:UniProtKB.
 GO:0009103; P:lipopolysaccharide biosynthetic process; ISS:UniProtKB.
 GO:0009243; P:O antigen biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR005888; dTDP_Gluc_deHydtase.
 IPR001509; Epimerase_deHydtase.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF01370; Epimerase 

SMART

  

PROSITE

  

PRINTS