CPLM-002389

Genbank Nucleotide ID

Carbon catabolite-derepressing protein kinase

Protein Synonyms/Alias

CAT1; CCR1; GLC2; PAS14; YDR477W; D8035.20

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Position	Peptide	Type	References
59	IGNYQIVKTLGEGSF	acetylation	[1]
68	LGEGSFGKVKLAYHT	acetylation	[1]
80	YHTTTGQKVALKIIN	ubiquitination	[2, 3]
148	DYIVQRDKMSEQEAR	ubiquitination	[3]
209	MTDGNFLKTSCGSPN	ubiquitination	[3]
360	SSTMGYEKDEIYESL	ubiquitination	[4]
435	QVDHETAKQHARRMA	ubiquitination	[3]
461	SPFMDQYKEEDSTVS	ubiquitination	[2]
492	QGSPAASKISPLVTK	ubiquitination	[3]
561	NKTNTNEKIPDLMKM	ubiquitination	[3]

[1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
[2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
[3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
[4] Identification, analysis, and prediction of protein ubiquitination sites.
Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]

Functional Description

Essential for release from glucose repression. It interacts and has functional relationship to the regulatory protein SNF4. Could phosphorylate CAT8. Phosphorylates histone H3 to form H3S10ph, which promotes H3K14ac formation, and which is required for transcriptional activation through TBP recruitment to the promoters.

DOMAIN 55 306 Protein kinase.
NP_BIND 61 69 ATP (By similarity).
ACT_SITE 177 177 Proton acceptor (By similarity).
BINDING 84 84 ATP (By similarity).
MOD_RES 210 210 Phosphothreonine; by autocatalysis.
MOD_RES 413 413 Phosphoserine.
MOD_RES 487 487 Phosphoserine.
MOD_RES 632 632 Phosphoserine.

3D-structure; ATP-binding; Carbohydrate metabolism; Complete proteome; Direct protein sequencing; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0031588; C:AMP-activated protein kinase complex; IDA:SGD.
GO:0000324; C:fungal-type vacuole; IPI:SGD.
GO:0005739; C:mitochondrion; IDA:SGD.
GO:0005641; C:nuclear envelope lumen; IDA:SGD.
GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
GO:0004679; F:AMP-activated protein kinase activity; IDA:SGD.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0042710; P:biofilm formation; IMP:SGD.
GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
GO:0007155; P:cell adhesion; IMP:SGD.
GO:0006995; P:cellular response to nitrogen starvation; IDA:SGD.
GO:0001403; P:invasive growth in response to glucose limitation; IMP:SGD.
GO:0017148; P:negative regulation of translation; IMP:SGD.
GO:1900436; P:positive regulation of filamentous growth of a population of unicellular organisms in response to starvation; IMP:SGD.
GO:0045722; P:positive regulation of gluconeogenesis; IMP:SGD.
GO:0007124; P:pseudohyphal growth; IMP:SGD.
GO:0001302; P:replicative cell aging; IMP:SGD.
GO:0007165; P:signal transduction; TAS:SGD.

IPR011009; Kinase-like_dom.
IPR000719; Prot_kinase_cat_dom.
IPR017441; Protein_kinase_ATP_BS.
IPR002290; Ser/Thr_dual-sp_kinase_dom.
IPR008271; Ser/Thr_kinase_AS.
IPR013896; Ubiquitin-assoc_dom.

PF00069; Pkinase
PF08587; UBA_2

PS00107; PROTEIN_KINASE_ATP
PS50011; PROTEIN_KINASE_DOM
PS00108; PROTEIN_KINASE_ST