UniProt Accession

 LYPA2_HUMAN; O95372; Q7Z4Z2 

Genbank Protein ID

 AF098668; AF090423; AL031295; BC017034; BC017193 

Genbank Nucleotide ID

 AAC72844.1; AAP97210.1; CAB40158.1; AAH17034.1; AAH17193.1 

Protein Name

 Acyl-protein thioesterase 2 

Protein Synonyms/Alias

 APT-2; Lysophospholipase II; LPL-II; LysoPLA II 

Gene Name

 LYPLA2 

Gene Synonyms/Alias

 APT2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
53	TIRLPHVKYICPHAP	ubiquitination	[1]
93	PEDEAGIKKAAENIK	ubiquitination	[2]
100	KKAAENIKALIEHEM	ubiquitination	[1, 2]
108	ALIEHEMKNGIPANR	ubiquitination	[1, 2, 3, 4]
164	QAANGSAKDLAILQC	ubiquitination	[1]
190	FGALTAEKLRSVVTP	ubiquitination	[1, 2]
203	TPARVQFKTYPGVMH	ubiquitination	[1]
221	PQEMAAVKEFLEKLL	ubiquitination	[1]
226	AVKEFLEKLLPPV**	ubiquitination	[1, 2, 4, 5]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 May hydrolyze fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has lysophospholipase activity (By similarity). Deacylates GAP43. 

Sequence Annotation

 ACT_SITE 122 122 Charge relay system (By similarity).
 ACT_SITE 176 176 Charge relay system (By similarity).
 ACT_SITE 210 210 Charge relay system (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Fatty acid metabolism; Hydrolase; Lipid metabolism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 231 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
 GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW. 

Interpro

 IPR003140; PLipase/COase/thioEstase. 

Pfam

 PF02230; Abhydrolase_2 

SMART

  

PROSITE

  

PRINTS