CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010687
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Fumarate hydratase, mitochondrial 
Protein Synonyms/Alias
 Fumarase; EF-3 
Gene Name
 Fh 
Gene Synonyms/Alias
 Fh1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
23ELKVPTDKYYGAQTVacetylation[1]
37VRSTMNFKIGGATERacetylation[1]
170VLLPGLQKLHDALSAacetylation[1]
180DALSAKSKEFAQVIKacetylation[1]
220QYAMVRIKAAMPRIYacetylation[1]
249TRIGFAEKVAAKVAAacetylation[1]
424NPHIGYDKAAKIAKTacetylation[1]
427IGYDKAAKIAKTAHKacetylation[1]
430DKAAKIAKTAHKNGSacetylation[1]
434KIAKTAHKNGSTLKEacetylation[1]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
  
Sequence Annotation
 REGION 173 176 B site (By similarity).
 REGION 183 185 Substrate binding (By similarity).
 BINDING 144 144 Substrate (By similarity).
 MOD_RES 63 63 N6-acetyllysine.
 MOD_RES 77 77 N6-acetyllysine (By similarity).
 MOD_RES 112 112 N6-acetyllysine.
 MOD_RES 289 289 N6-acetyllysine (By similarity).
 MOD_RES 474 474 N6-acetyllysine.  
Keyword
 Acetylation; Alternative initiation; Complete proteome; Cytoplasm; Direct protein sequencing; Lyase; Mitochondrion; Reference proteome; Transit peptide; Tricarboxylic acid cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 507 AA 
Protein Sequence
MASQNSFRVE FDTFGELKVP TDKYYGAQTV RSTMNFKIGG ATERMPIPVI QAFGILKRAA 60
AEVNQEYGLD PKIASAIMKA ADEVAEGKLN DHFPLVVWQT GSGTQTNMNV NEVISNRAIE 120
MLGGELGSKK PVHPNDHVNK SQSSNDTFPT AMHIAAAVEV HKVLLPGLQK LHDALSAKSK 180
EFAQVIKIGR THTQDAVPLT LGQEFSGYVQ QVQYAMVRIK AAMPRIYELA AGGTAVGTGL 240
NTRIGFAEKV AAKVAALTGL PFVTAPNKFE ALAAHDALVE LSGAMNTAAC SLMKIANDIR 300
FLGSGPRSGL GELILPENEP GSSIMPGKVN PTQCEAMTMV AAQVMGNHVA VTVGGSNGHF 360
ELNVFKPMMI KNVLHSARLL GDASVSFTDN CVVGIQANTE RINKLMNESL MLVTALNPHI 420
GYDKAAKIAK TAHKNGSTLK ETAIELGYLT AEQFDEWVKP KDMLGPK 467 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0045239; C:tricarboxylic acid cycle enzyme complex; IEA:InterPro.
 GO:0004333; F:fumarate hydratase activity; IEA:EC.
 GO:0006106; P:fumarate metabolic process; IEA:InterPro.
 GO:0048873; P:homeostasis of number of cells within a tissue; IMP:MGI.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway. 
Interpro
 IPR005677; Fum_hydII.
 IPR024083; Fumarase/histidase_N.
 IPR018951; Fumarase_C_C.
 IPR000362; Fumarate_lyase.
 IPR020557; Fumarate_lyase_CS.
 IPR022761; Fumarate_lyase_N.
 IPR008948; L-Aspartase-like. 
Pfam
 PF10415; FumaraseC_C
 PF00206; Lyase_1 
SMART
  
PROSITE
 PS00163; FUMARATE_LYASES 
PRINTS
 PR00149; FUMRATELYASE.