CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005065
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase VPS15 
Protein Synonyms/Alias
 Golgi-retention defective mutant protein 8; Vacuolar protein sorting-associated protein 15 
Gene Name
 VPS15 
Gene Synonyms/Alias
 GRD8; VAC4; VPL19; YBR097W; YBR0825 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
205APERFNSKLYQDGKSubiquitination[1]
211SKLYQDGKSNNGRLTubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Serine/threonine-protein kinase required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Is also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). By regulating VPS34 kinase activity, VPS15 appears to be essential for the efficient delivery of soluble hydrolases to the yeast vacuole. 
Sequence Annotation
 DOMAIN 27 300 Protein kinase.
 REPEAT 460 497 HEAT 1.
 REPEAT 576 613 HEAT 2.
 REPEAT 615 652 HEAT 3.
 REPEAT 654 691 HEAT 4.
 REPEAT 1078 1118 WD 1.
 REPEAT 1126 1165 WD 2.
 REPEAT 1229 1268 WD 3.
 REPEAT 1275 1315 WD 4.
 REPEAT 1344 1382 WD 5.
 REPEAT 1422 1453 WD 6.
 NP_BIND 33 41 ATP (By similarity).
 ACT_SITE 147 147 Proton acceptor.
 BINDING 54 54 ATP (By similarity).
 LIPID 2 2 N-myristoyl glycine.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Endosome; Golgi apparatus; Kinase; Lipoprotein; Membrane; Myristate; Nucleotide-binding; Phosphoprotein; Protein transport; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase; Transport; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1454 AA 
Protein Sequence
MGAQLSLVVQ ASPSIAIFSY IDVLEEVHYV SQLNSSRFLK TCKALDPNGE IVIKVFIKPK 60
DQYSLRPFLQ RIRAQSFKLG QLPHVLNYSK LIETNRAGYM IRQHLKNNLY DRLSLRPYLQ 120
DIELKFIAFQ LLNALKDIHN LNIVHGDIKT ENILVTSWNW CILTDFAAFI KPVYLPEDNP 180
GEFLFYFDTS KRRTCYLAPE RFNSKLYQDG KSNNGRLTKE MDIFSLGCVI AEIFAEGRPI 240
FNLSQLFKYK SNSYDVNREF LMEEMNSTDL RNLVLDMIQL DPSKRLSCDE LLNKYRGIFF 300
PDYFYTFIYD YFRNLVTMTT STPISDNTCT NSTLEDNVKL LDETTEKIYR DFSQICHCLD 360
FPLIKDGGEI GSDPPILESY KIEIEISRFL NTNLYFPQNY HLVLQQFTKV SEKIKSVKEE 420
CALLFISYLS HSIRSIVSTA TKLKNLELLA VFAQFVSDEN KIDRVVPYFV CCFEDSDQDV 480
QALSLLTLIQ VLTSVRKLNQ LNENIFVDYL LPRLKRLLIS NRQNTNYLRI VFANCLSDLA 540
IIINRFQEFT FAQHCNDNSM DNNTEIMESS TKYSAKLIQS VEDLTVSFLT DNDTYVKMAL 600
LQNILPLCKF FGRERTNDII LSHLITYLND KDPALRVSLI QTISGISILL GTVTLEQYIL 660
PLLIQTITDS EELVVISVLQ SLKSLFKTGL IRKKYYIDIS KTTSPLLLHP NNWIRQFTLM 720
IIIEIINKLS KAEVYCILYP IIRPFFEFDV EFNFKSMISC CKQPVSRSVY NLLCSWSVRA 780
SKSLFWKKII TNHVDSFGNN RIEFITKNYS SKNYGFNKRD TKSSSSLKGI KTSSTVYSHD 840
NKEIPLTAED RNWIDKFHII GLTEKDIWKI VALRGYVIRT ARVMAANPDF PYNNSNYRPL 900
VQNSPPNLNL TNIMPRNIFF DVEFAEESTS EGQDSNLENQ QIYKYDESEK DSNKLNINGS 960
KQLSTVMDIN GSLIFKNKSI ATTTSNLKNV FVQLEPTSYH MHSPNHGLKD NANVKPERKV 1020
VVSNSYEGDV ESIEKFLSTF KILPPLRDYK EFGPIQEIVR SPNMGNLRGK LIATLMENEP 1080
NSITSSAVSP GETPYLITGS DQGVIKIWNL KEIIVGEVYS SSLTYDCSST VTQITMIPNF 1140
DAFAVSSKDG QIIVLKVNHY QQESEVKFLN CECIRKINLK NFGKNEYAVR MRAFVNEEKS 1200
LLVALTNLSR VIIFDIRTLE RLQIIENSPR HGAVSSICID EECCVLILGT TRGIIDIWDI 1260
RFNVLIRSWS FGDHAPITHV EVCQFYGKNS VIVVGGSSKT FLTIWNFVKG HCQYAFINSD 1320
EQPSMEHFLP IEKGLEELNF CGIRSLNALS TISVSNDKIL LTDEATSSIV MFSLNELSSS 1380
KAVISPSRFS DVFIPTQVTA NLTMLLRKMK RTSTHSVDDS LYHHDIINSI STCEVDETPL 1440
LVACDNSGLI GIFQ 1454 
Gene Ontology
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0071561; C:nucleus-vacuole junction; IDA:SGD.
 GO:0034271; C:phosphatidylinositol 3-kinase complex I; IDA:SGD.
 GO:0034272; C:phosphatidylinositol 3-kinase complex II; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:SGD.
 GO:0043130; F:ubiquitin binding; IDA:SGD.
 GO:0048017; P:inositol lipid-mediated signaling; IMP:SGD.
 GO:0045324; P:late endosome to vacuole transport; IMP:SGD.
 GO:0016236; P:macroautophagy; IMP:SGD.
 GO:0030242; P:peroxisome degradation; IMP:SGD.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IMP:SGD.
 GO:0045053; P:protein retention in Golgi apparatus; IMP:SGD.
 GO:0006623; P:protein targeting to vacuole; IMP:SGD.
 GO:0000011; P:vacuole inheritance; IMP:SGD. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR021133; HEAT_type_2.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR008271; Ser/Thr_kinase_AS.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00069; Pkinase 
SMART
 SM00320; WD40 
PROSITE
 PS50077; HEAT_REPEAT
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS