| Tag | Content |
|---|
| CPLM ID | CPLM-023677 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Fanconi-associated nuclease 1 |
Protein Synonyms/Alias | FANCD2/FANCI-associated nuclease 1; Myotubularin-related protein 15 |
Gene Name | FAN1 |
Gene Synonyms/Alias | KIAA1018; MTMR15 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 137 | KSNDVVCKNQDELRN | ubiquitination | [1] | | 493 | LVNPNGQKQQLVDAF | ubiquitination | [1] | | 502 | QLVDAFLKLAKQRSV | ubiquitination | [1] | | 592 | PSYTINRKTHIFQDR | ubiquitination | [1] | | 628 | NGNWEEAKELAQCAK | ubiquitination | [1] | | 720 | LNLHQHLKRLEPTIK | ubiquitination | [1] | | 727 | KRLEPTIKCITEGLA | ubiquitination | [1] | | 977 | HFKLVEVKGPNDRLS | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL- induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap- specific enzymes to unhook the ICL by cleaving the lagging-strand template. |
Sequence Annotation | DOMAIN 895 1007 VRR-NUC.
ZN_FING 41 67 UBZ-type.
MOTIF 14 22 D-box.
MOTIF 212 214 KEN box. |
Keyword | Alternative splicing; Coiled coil; Complete proteome; Disease mutation; DNA damage; DNA repair; Endonuclease; Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease; Nucleus; Polymorphism; Reference proteome; Ubl conjugation; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1017 AA |
Protein Sequence | MMSEGKPPDK KRPRRSLSIS KNKKKASNSI ISCFNNAPPA KLACPVCSKM VPRYDLNRHL 60
DEMCANNDFV QVDPGQVGLI NSNVSMVDLT SVTLEDVTPK KSPPPKTNLT PGQSDSAKRE 120
VKQKISPYFK SNDVVCKNQD ELRNRSVKVI CLGSLASKLS RKYVKAKKSI DKDEEFAGSS 180
PQSSKSTVVK SLIDNSSEIE DEDQILENSS QKENVFKCDS LKEECIPEHM VRGSKIMEAE 240
SQKATRECEK SALTPGFSDN AIMLFSPDFT LRNTLKSTSE DSLVKQECIK EVVEKREACH 300
CEEVKMTVAS EAKIQLSDSE AKSHSSADDA SAWSNIQEAP LQDDSCLNND IPHSIPLEQG 360
SSCNGPGQTT GHPYYLRSFL VVLKTVLENE DDMLLFDEQE KGIVTKFYQL SATGQKLYVR 420
LFQRKLSWIK MTKLEYEEIA LDLTPVIEEL TNAGFLQTES ELQELSEVLE LLSAPELKSL 480
AKTFHLVNPN GQKQQLVDAF LKLAKQRSVC TWGKNKPGIG AVILKRAKAL AGQSVRICKG 540
PRAVFSRILL LFSLTDSMED EDAACGGQGQ LSTVLLVNLG RMEFPSYTIN RKTHIFQDRD 600
DLIRYAAATH MLSDISSAMA NGNWEEAKEL AQCAKRDWNR LKNHPSLRCH EDLPLFLRCF 660
TVGWIYTRIL SRFVEILQRL HMYEEAVREL ESLLSQRIYC PDSRGRWWDR LALNLHQHLK 720
RLEPTIKCIT EGLADPEVRT GHRLSLYQRA VRLRESPSCK KFKHLFQQLP EMAVQDVKHV 780
TITGRLCPQR GMCKSVFVME AGEAADPTTV LCSVEELALA HYRRSGFDQG IHGEGSTFST 840
LYGLLLWDII FMDGIPDVFR NACQAFPLDL CTDSFFTSRR PALEARLQLI HDAPEESLRA 900
WVAATWHEQE GRVASLVSWD RFTSLQQAQD LVSCLGGPVL SGVCRHLAAD FRHCRGGLPD 960
LVVWNSQSRH FKLVEVKGPN DRLSHKQMIW LAELQKLGAE VEVCHVVAVG AKSQSLS 1017 |
Gene Ontology | GO:0005634; C:nucleus; IDA:UniProtKB. GO:0008409; F:5'-3' exonuclease activity; IDA:UniProtKB. GO:0017108; F:5'-flap endonuclease activity; IDA:UniProtKB. GO:0003677; F:DNA binding; IEA:InterPro. GO:0000287; F:magnesium ion binding; TAS:UniProtKB. GO:0004528; F:phosphodiesterase I activity; IEA:EC. GO:0043130; F:ubiquitin binding; IDA:UniProtKB. GO:0000724; P:double-strand break repair via homologous recombination; IMP:UniProtKB. GO:0033683; P:nucleotide-excision repair, DNA incision; IDA:UniProtKB. |
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