UniProt Accession

 PURA_MYCTU; P65880; L0T6F4; O08381 

Genbank Protein ID

 AE000516; AL123456 

Genbank Nucleotide ID

 AAK44594.1; CCP43087.1 

Protein Name

 Adenylosuccinate synthetase 

Protein Synonyms/Alias

 AMPSase; AdSS; IMP--aspartate ligase 

Gene Name

 purA 

Gene Synonyms/Alias

 Rv0357c; MT0373; MTCY13E10.19c 

Created Date

 July 27, 2013 

Organism

 Mycobacterium tuberculosis 

NCBI Taxa ID

 1773 

Lysine Modification

Position	Peptide	Type	References
292	EHGEYLSKTGREFGV	pupylation	[1]

Reference

 [1] Prokaryotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis [corrected] .
 Festa RA, McAllister F, Pearce MJ, Mintseris J, Burns KE, Gygi SP, Darwin KH.
 PLoS One. 2010 Jan 6;5(1):e8589. [PMID: 20066036] 

Functional Description

 Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity). 

Sequence Annotation

 NP_BIND 12 18 GTP (By similarity).
 NP_BIND 40 42 GTP (By similarity).
 NP_BIND 331 333 GTP (By similarity).
 NP_BIND 413 415 GTP (By similarity).
 REGION 13 16 IMP binding (By similarity).
 REGION 38 41 IMP binding (By similarity).
 REGION 299 305 Substrate binding (By similarity).
 ACT_SITE 13 13 Proton acceptor (By similarity).
 ACT_SITE 41 41 Proton donor (By similarity).
 METAL 13 13 Magnesium (By similarity).
 METAL 40 40 Magnesium; via carbonyl oxygen (By
 BINDING 129 129 IMP (By similarity).
 BINDING 143 143 IMP; shared with dimeric partner (By
 BINDING 224 224 IMP (By similarity).
 BINDING 239 239 IMP (By similarity).
 BINDING 303 303 IMP (By similarity).
 BINDING 305 305 GTP (By similarity).  

Keyword

 Complete proteome; Cytoplasm; GTP-binding; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Purine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 432 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004019; F:adenylosuccinate synthase activity; IEA:HAMAP.
 GO:0005525; F:GTP binding; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0044208; P:'de novo' AMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0040007; P:growth; IMP:MTBBASE. 

Interpro

 IPR018220; Adenylosuccinate_synthase_AS.
 IPR001114; Adenylosuccinate_synthetase.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00709; Adenylsucc_synt 

SMART

 SM00788; Adenylsucc_synt 

PROSITE

 PS01266; ADENYLOSUCCIN_SYN_1
 PS00513; ADENYLOSUCCIN_SYN_2 

PRINTS