UniProt Accession

 PSB1_YEAST; P38624; D6VWH4 

Genbank Protein ID

 X78991; Z49276; Z49277; X87611; X86020; S78566; BK006943 

Genbank Nucleotide ID

 CAA55591.1; CAA89290.1; CAA89292.1; CAA60921.1; CAA60015.1; AAB34629.1; DAA08790.1 

Protein Name

 Proteasome subunit beta type-1 

Protein Synonyms/Alias

 Macropain subunit PRE3; Multicatalytic endopeptidase complex subunit PRE3; Proteasome component PRE3; Proteinase YSCE subunit PRE3 

Gene Name

 PRE3 

Gene Synonyms/Alias

 YJL001W; J1407 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
52	IANRVTDKLTRVHDK	ubiquitination	[1]
59	KLTRVHDKIWCCRSG	ubiquitination	[1]
159	FIYGYCDKNFRENMS	ubiquitination	[1]
183	HSLSQAIKWDGSSGG	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity. PRE3 and PRE4 are necessary for the peptidyl-glutamyl-peptide- hydrolyzing activity. 

Sequence Annotation

 ACT_SITE 20 20 Nucleophile.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Hydrolase; Nucleus; Protease; Proteasome; Reference proteome; Threonine protease; Zymogen. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 215 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IC:SGD.
 GO:0005634; C:nucleus; IC:SGD.
 GO:0019774; C:proteasome core complex, beta-subunit complex; IDA:SGD.
 GO:0034515; C:proteasome storage granule; IDA:SGD.
 GO:0004175; F:endopeptidase activity; IMP:SGD.
 GO:0004298; F:threonine-type endopeptidase activity; IEA:UniProtKB-KW.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IDA:SGD.
 GO:0010499; P:proteasomal ubiquitin-independent protein catabolic process; IDA:SGD. 

Interpro

 IPR000243; Pept_T1A_subB.
 IPR016050; Proteasome_bsu_CS.
 IPR001353; Proteasome_sua/b.
 IPR023333; Proteasome_suB-type. 

Pfam

 PF00227; Proteasome 

SMART

  

PROSITE

 PS00854; PROTEASOME_B_1
 PS51476; PROTEASOME_B_2 

PRINTS

 PR00141; PROTEASOME.