CPLM-008372

Genbank Nucleotide ID

Tyrosine-protein kinase Blk

Protein Synonyms/Alias

B lymphocyte kinase; p55-Blk

Homo sapiens (Human)

Position	Peptide	Type	References
16	PDKEKPIKEKDKGQW	acetylation	[1]
144	QLLAPINKAGSFLIR	ubiquitination	[2]
272	KVAIKTLKEGTMSPE	ubiquitination	[2]
325	GCLLDFLKTDEGSRL	ubiquitination	[2]

[1] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr- 207'. Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose.

DOMAIN 58 118 SH3.
DOMAIN 124 220 SH2.
DOMAIN 241 494 Protein kinase.
NP_BIND 247 255 ATP (By similarity).
ACT_SITE 360 360 Proton acceptor (By similarity).
BINDING 269 269 ATP (By similarity).
MOD_RES 389 389 Phosphotyrosine; by autocatalysis (By
LIPID 2 2 N-myristoyl glycine (By similarity).

ATP-binding; Cell membrane; Complete proteome; Diabetes mellitus; Kinase; Lipoprotein; Membrane; Myristate; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IDA:UniProtKB.
GO:0050853; P:B cell receptor signaling pathway; IDA:UniProtKB.
GO:0007243; P:intracellular protein kinase cascade; TAS:ProtInc.
GO:0032024; P:positive regulation of insulin secretion; IMP:UniProtKB.

IPR011009; Kinase-like_dom.
IPR000719; Prot_kinase_cat_dom.
IPR017441; Protein_kinase_ATP_BS.
IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
IPR000980; SH2.
IPR001452; SH3_domain.
IPR020635; Tyr_kinase_cat_dom.

PF07714; Pkinase_Tyr
PF00017; SH2
PF00018; SH3_1

SM00252; SH2
SM00326; SH3
SM00219; TyrKc

PS00107; PROTEIN_KINASE_ATP
PS50011; PROTEIN_KINASE_DOM
PS00109; PROTEIN_KINASE_TYR
PS50001; SH2
PS50002; SH3

PR00401; SH2DOMAIN.
PR00452; SH3DOMAIN.
PR00109; TYRKINASE.