CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-034258
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Acyl-coenzyme A synthetase ACSM1, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 Acsm1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
179ASECPDLKTKLVVSDacetylation[1, 2, 3]
179ASECPDLKTKLVVSDsuccinylation[2]
179ASECPDLKTKLVVSDubiquitination[4]
200LDFCSLIKSASPDHTacetylation[1, 2, 3, 5, 6]
200LDFCSLIKSASPDHTsuccinylation[2]
210SPDHTCIKSKMKDPMacetylation[1, 3]
210SPDHTCIKSKMKDPMubiquitination[4]
214TCIKSKMKDPMAIFFacetylation[5]
214TCIKSKMKDPMAIFFubiquitination[4]
230SGTTGYPKMAKHNQGubiquitination[4]
233TGYPKMAKHNQGLAFacetylation[2]
233TGYPKMAKHNQGLAFsuccinylation[2]
233TGYPKMAKHNQGLAFubiquitination[4]
324YRMVLQQKTSNLRFPacetylation[2]
324YRMVLQQKTSNLRFPsuccinylation[2]
324YRMVLQQKTSNLRFPubiquitination[4]
352PEEYEQWKQRTGLSIacetylation[1, 2, 3, 5]
352PEEYEQWKQRTGLSIsuccinylation[2]
352PEEYEQWKQRTGLSIubiquitination[4]
474AVVSSPDKDRGEVVKacetylation[1, 2, 3]
474AVVSSPDKDRGEVVKsuccinylation[2]
481KDRGEVVKAFIVLNPacetylation[3]
500HDQEQLIKELQHHVKacetylation[1, 3]
507KELQHHVKSVTAPYKacetylation[1, 2, 3, 7]
507KELQHHVKSVTAPYKsuccinylation[2]
507KELQHHVKSVTAPYKubiquitination[4]
514KSVTAPYKYPRKVEFacetylation[1, 2, 3]
514KSVTAPYKYPRKVEFsuccinylation[2]
518APYKYPRKVEFVSELacetylation[1]
Reference
 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [6] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [7] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 531 AA 
Protein Sequence
MQWLKSFQIC KVLQGFSLSP TQLHRRLFSR VGAPRWNDHD SPEEFNFASD VLDYWAQMEE 60
EGKRGPSPAF WWVNGQGDEI KWSFRKLRDL TCRTANVFEQ ICGLQQGDHL ALILPRVPEW 120
WLVTVGCMRT GIIFMPGTTQ LKAKDILYRI QISRAKAIVT TASLVPEVES VASECPDLKT 180
KLVVSDHSHE GWLDFCSLIK SASPDHTCIK SKMKDPMAIF FTSGTTGYPK MAKHNQGLAF 240
RSYIPSCRKL LKLKTSDILW CMSDPGWILA TVGCLIEPWT SGCTVFIHHL PQFDPKVIVE 300
VLFKYPITQC LAAPGVYRMV LQQKTSNLRF PTLEHCTTGG ESLLPEEYEQ WKQRTGLSIH 360
EVYGQSETII DEKGNILPPN TEGYIGIRIK PTRPLGLFME YENSPESTSE VECGDFYNSG 420
DRATIDEEGY IWFLGRGDDV INASGYRIGP VEVENALAEH PAVAESAVVS SPDKDRGEVV 480
KAFIVLNPEF LSHDQEQLIK ELQHHVKSVT APYKYPRKVE FVSELPKTVT G 531 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0015645; F:fatty acid ligase activity; IDA:MGI.
 GO:0006633; P:fatty acid biosynthetic process; IDA:MGI. 
Interpro
 IPR020845; AMP-binding_CS.
 IPR000873; AMP-dep_Synth/Lig.
 IPR025110; DUF4009. 
Pfam
 PF00501; AMP-binding
 PF13193; DUF4009 
SMART
  
PROSITE
 PS00455; AMP_BINDING 
PRINTS