CPLM-014371

Genbank Nucleotide ID

3 beta-hydroxysteroid dehydrogenase type 5

Protein Synonyms/Alias

3 beta-hydroxysteroid dehydrogenase type V; 3-beta-HSD V; 3-beta-hydroxy-5-ene steroid dehydrogenase; NADPH-dependent 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; Progesterone reductase

Mus musculus (Mouse)

Position	Peptide	Type	References
42	LFRTFGRKHEEELSK	acetylation	[1]
49	KHEEELSKLQTKAKV	acetylation	[2]
49	KHEEELSKLQTKAKV	ubiquitination	[3]
60	KAKVRVLKGDILDAQ	acetylation	[1, 2, 4, 5, 6]
60	KAKVRVLKGDILDAQ	succinylation	[5]
60	KAKVRVLKGDILDAQ	ubiquitination	[3]
70	ILDAQCLKRACQGMS	acetylation	[2, 4, 6]
70	ILDAQCLKRACQGMS	ubiquitination	[3]
164	YSKRMAEKAVLATNG	acetylation	[6]
164	YSKRMAEKAVLATNG	ubiquitination	[3]
175	ATNGRLLKNGGTLHT	acetylation	[2, 4, 6]
175	ATNGRLLKNGGTLHT	ubiquitination	[3]
207	TTVKTALKNNSIIKK	acetylation	[4]
207	TTVKTALKNNSIIKK	ubiquitination	[3]
244	ARSLQDPKKSPSIQG	acetylation	[4]
350	LVSWEEAKQKTSEWI	acetylation	[2, 4, 6]
350	LVSWEEAKQKTSEWI	ubiquitination	[3]
352	SWEEAKQKTSEWIGT	acetylation	[4, 6]
352	SWEEAKQKTSEWIGT	ubiquitination	[3]
362	EWIGTLVKQHRETLH	acetylation	[1, 2, 4, 6]

[1] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
[2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]

Functional Description

The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD V can only catalyze the conversion of dihydrotestosterone to 5 alpha- androstanediol in the presence of the cofactor NADPH. Does not function as a isomerase.

ACT_SITE 155 155 Proton acceptor (By similarity).
BINDING 159 159 NAD (By similarity).
MOD_RES 42 42 N6-acetyllysine.
MOD_RES 60 60 N6-acetyllysine.
MOD_RES 362 362 N6-acetyllysine.

Acetylation; Complete proteome; Endoplasmic reticulum; Membrane; Mitochondrion; NADP; Oxidoreductase; Reference proteome; Steroidogenesis; Transmembrane; Transmembrane helix.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase activity; IEA:InterPro.
GO:0035634; P:response to stilbenoid; IEP:UniProtKB.
GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-UniPathway.

IPR002225; 3Beta_OHSteriod_DH/Estase.
IPR016040; NAD(P)-bd_dom.