CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014082
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Telomere-associated protein RIF1 
Protein Synonyms/Alias
 Rap1-interacting factor 1 homolog 
Gene Name
 RIF1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43RMTGEEGKEVITEIEubiquitination[1]
109SKLNDTIKNSDKNVRubiquitination[1]
125RALWVISKQTFPSEVubiquitination[2]
198LVVHSAQKVHLRGATubiquitination[1, 2]
251KNETYVLKLWPLFVKubiquitination[2]
315PDILCSAKRLKLLMQubiquitination[1]
318LCSAKRLKLLMQPLSubiquitination[1]
702RFPVATMKTLLRTWSubiquitination[2, 3]
799KKNEPLGKLTSLFKLacetylation[4]
929HKNKQIRKQSAQFWNubiquitination[1]
952LVYPEELKPVLTQAKubiquitination[1]
961VLTQAKQKFLLLLPGubiquitination[1]
1274QREGTFSKSDSEKIVacetylation[5]
1744EKSQPQEKSLIGLKNubiquitination[6]
1763DVEISETKKADVQAPubiquitination[3]
2249TSPTTQSKHNTTSAKacetylation[5]
2328LIRAKNIKTIGDLSTubiquitination[2]
2342TLTASEIKTLPIRSPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
 Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S- phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints. 
Sequence Annotation
 REGION 1924 2472 Interaction with condensed chromosomes in
 MOD_RES 402 402 Phosphoserine.
 MOD_RES 409 409 Phosphothreonine.
 MOD_RES 782 782 Phosphoserine.
 MOD_RES 979 979 Phosphoserine.
 MOD_RES 1047 1047 Phosphothreonine.
 MOD_RES 1162 1162 Phosphoserine.
 MOD_RES 1236 1236 Phosphoserine.
 MOD_RES 1238 1238 Phosphoserine.
 MOD_RES 1422 1422 Phosphoserine.
 MOD_RES 1454 1454 Phosphoserine.
 MOD_RES 1513 1513 Phosphoserine.
 MOD_RES 1518 1518 Phosphothreonine.
 MOD_RES 1542 1542 Phosphoserine.
 MOD_RES 1552 1552 Phosphoserine.
 MOD_RES 1554 1554 Phosphoserine.
 MOD_RES 1576 1576 Phosphoserine.
 MOD_RES 1579 1579 Phosphoserine.
 MOD_RES 1616 1616 Phosphoserine.
 MOD_RES 1688 1688 Phosphoserine.
 MOD_RES 1693 1693 Phosphoserine.
 MOD_RES 1806 1806 Phosphothreonine.
 MOD_RES 1810 1810 Phosphoserine.
 MOD_RES 1873 1873 Phosphoserine.
 MOD_RES 1876 1876 Phosphoserine.
 MOD_RES 1971 1971 Phosphoserine.
 MOD_RES 2144 2144 Phosphoserine.
 MOD_RES 2161 2161 Phosphoserine.
 MOD_RES 2167 2167 Phosphothreonine.
 MOD_RES 2172 2172 Phosphoserine.
 MOD_RES 2176 2176 Phosphoserine.
 MOD_RES 2196 2196 Phosphoserine.
 MOD_RES 2205 2205 Phosphoserine.
 MOD_RES 2260 2260 Phosphoserine.
 MOD_RES 2339 2339 Phosphoserine.
 MOD_RES 2391 2391 Phosphoserine.
 MOD_RES 2393 2393 Phosphoserine.
 MOD_RES 2465 2465 Phosphoserine.
 MOD_RES 2471 2471 Phosphoserine.  
Keyword
 Alternative splicing; Cell cycle; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; DNA damage; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Telomere. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2472 AA 
Protein Sequence
MTARGQSPLA PLLETLEDPS ASHGGQTDAY LTLTSRMTGE EGKEVITEIE KKLPRLYKVL 60
KTHISSQNSE LSSAALQALG FCLYNPKITS ELSEANALEL LSKLNDTIKN SDKNVRTRAL 120
WVISKQTFPS EVVGKMVSSI IDSLEILFNK GETHSAVVDF EALNVIVRLI EQAPIQMGEE 180
AVRWAKLVIP LVVHSAQKVH LRGATALEMG MPLLLQKQQE IASITEQLMT TKLISELQKL 240
FMSKNETYVL KLWPLFVKLL GRTLHRSGSF INSLLQLEEL GFRSGAPMIK KIAFIAWKSL 300
IDNFALNPDI LCSAKRLKLL MQPLSSIHVR TETLALTKLE VWWYLLMRLG PHLPANFEQV 360
CVPLIQSTIS IDSNASPQGN SCHVATSPGL NPMTPVHKGA SSPYGAPGTP RMNLSSNLGG 420
MATIPSIQLL GLEMLLHFLL GPEALSFAKQ NKLVLSLEPL EHPLISSPSF FSKHANTLIT 480
AVHDSFVAVG KDAPDVVVSA IWKELISLVK SVTESGNKKE KPGSEVLTLL LKSLESIVKS 540
EVFPVSKTLV LMEITIKGLP QKVLGSPAYQ VANMDILNGT PALFLIQLIF NNFLECGVSD 600
ERFFLSLESL VGCVLSGPTS PLAFSDSVLN VINQNAKQLE NKEHLWKMWS VIVTPLTELI 660
NQTNEVNQGD ALEHNFSAIY GALTLPVNHI FSEQRFPVAT MKTLLRTWSE LYRAFARCAA 720
LVATAEENLC CEELSSKIMS SLEDEGFSNL LFVDRIIYII TVMVDCIDFS PYNIKYQPKV 780
KSPQRPSDWS KKKNEPLGKL TSLFKLIVKV IYSFHTLSFK EAHSDTLFTI GNSITGIISS 840
VLGHISLPSM IRKIFATLTR PLALFYENSK LDEVPKVYSC LNNKLEKLLG EIIACLQFSY 900
TGTYDSELLE QLSPLLCIIF LHKNKQIRKQ SAQFWNATFA KVMMLVYPEE LKPVLTQAKQ 960
KFLLLLPGLE TVEMMEESSG PYSDGTENSQ LNVKISGMER KSNGKRDSFL AQTKNKKENM 1020
KPAAKLKLES SSLKVKGEIL LEEEKSTDFV FIPPEGKDAK ERILTDHQKE VLKTKRCDIP 1080
AMYNNLDVSQ DTLFTQYSQE EPMEIPTLTR KPKEDSKMMI TEEQMDSDIV IPQDVTEDCG 1140
MAEHLEKSSL SNNECGSLDK TSPEMSNSNN DERKKALISS RKTSTECASS TENSFVVSSS 1200
SVSNTTVAGT PPYPTSRRQT FITLEKFDGS ENRPFSPSPL NNISSTVTVK NNQETMIKTD 1260
FLPKAKQREG TFSKSDSEKI VNGTKRSSRR AGKAEQTGNK RSKPLMRSEP EKNTEESVEG 1320
IVVLENNPPG LLNQTECVSD NQVHLSESTM EHDNTKLKAA TVENAVLLET NTVEEKNVEI 1380
NLESKENTPP VVISADQMVN EDSQVQITPN QKTLRRSSRR RSEVVESTTE SQDKENSHQK 1440
KERRKEEEKP LQKSPLHIKD DVLPKQKLIA EQTLQENLIE KGSNLHEKTL GETSANAETE 1500
QNKKKADPEN IKSEGDGTQD IVDKSSEKLV RGRTRYQTRR ASQGLLSSIE NSESDSSEAK 1560
EEGSRKKRSG KWKNKSNESV DIQDQEEKVV KQECIKAENQ SHDYKATSEE DVSIKSPICE 1620
KQDESNTVIC QDSTVTSDLL QVPDDLPNVC EEKNETSKYA EYSFTSLPVP ESNLRTRNAI 1680
KRLHKRDSFD NCSLGESSKI GISDISSLSE KTFQTLECQH KRSRRVRRSK GCDCCGEKSQ 1740
PQEKSLIGLK NTENNDVEIS ETKKADVQAP VSPSETSQAN PYSEGQFLDE HHSVNFHLGL 1800
KEDNDTINDS LIVSETKSKE NTMQESLPSG IVNFREEICD MDSSEAMSLE SQESPNENFK 1860
TVGPCLGDSK NVSQESLETK EEKPEETPKM ELSLENVTVE GNACKVTESN LEKAKTMELN 1920
VGNEASFHGQ ERTKTGISEE AAIEENKRND DSEADTAKLN AKEVATEEFN SDISLSDNTT 1980
PVKLNAQTEI SEQTAAGELD GGNDVSDLHS SEETNTKMKN NEEMMIGEAM AETGHDGETE 2040
NEGITTKTSK PDEAETNMLT AEMDNFVCDT VEMSTEEGII DANKTETNTE YSKSEEKLDN 2100
NQMVMESDIL QEDHHTSQKV EEPSQCLASG TAISELIIED NNASPQKLRE LDPSLVSAND 2160
SPSGMQTRCV WSPLASPSTS ILKRGLKRSQ EDEISSPVNK VRRVSFADPI YQAGLADDID 2220
RRCSIVRSHS SNSSPIGKSV KTSPTTQSKH NTTSAKGFLS PGSRSPKFKS SKKCLISEMA 2280
KESIPCPTES VYPPLVNCVA PVDIILPQIT SNMWARGLGQ LIRAKNIKTI GDLSTLTASE 2340
IKTLPIRSPK VSNVKKALRI YHEQQVKTRG LEEIPVFDIS EKTVNGIENK SLSPDEERLV 2400
SDIIDPVALE IPLSKNLLAQ ISALALQLDS EDLHNYSGSQ LFEMHEKLSC MANSVIKNLQ 2460
SRWRSPSHEN SI 2472 
Gene Ontology
 GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0001939; C:female pronucleus; IEA:Compara.
 GO:0001940; C:male pronucleus; IEA:Compara.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0006974; P:response to DNA damage stimulus; IEA:UniProtKB-KW.
 GO:0019827; P:stem cell maintenance; IEA:Compara. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR022031; Rif1_N. 
Pfam
 PF12231; Rif1_N 
SMART
  
PROSITE
  
PRINTS