UniProt Accession

 RFC3_YEAST; P38629; D6W0Q3 

Genbank Protein ID

 L18755; U26029; U23084; Z71566; BK006947 

Genbank Nucleotide ID

 AAA34969.1; AAC49062.1; AAC49110.1; CAA96207.1; DAA10269.1 

Protein Name

 Replication factor C subunit 3 

Protein Synonyms/Alias

 Replication factor C3; Activator 1 40 kDa subunit 

Gene Name

 RFC3 

Gene Synonyms/Alias

 YNL290W; N0533 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
337	SFENETVKANV****	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Component of ATP-dependent clamp loader (RFC and RFC- like) complexes for DNA clamps, such as the POL30/PCNA homotrimer and the checkpoint clamp DDC1:MEC3:RAD17 complex. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Component of the replication factor C (RFC or activator 1) complex which loads POL30/PCNA and acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair. RFC3 supplies a catalytic site to the ATP site of RFC4. 

Sequence Annotation

 NP_BIND 16 19 ATP.
 NP_BIND 53 61 ATP.
 BINDING 20 20 ATP.
 BINDING 28 28 ATP; via amide nitrogen and carbonyl
 BINDING 148 148 ATP.
 BINDING 206 206 ATP.  

Keyword

 3D-structure; ATP-binding; Cell cycle; Complete proteome; DNA replication; DNA-binding; Nucleotide-binding; Nucleus; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 340 AA 

Protein Sequence

Gene Ontology

 GO:0031390; C:Ctf18 RFC-like complex; IPI:SGD.
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005663; C:DNA replication factor C complex; IDA:SGD.
 GO:0031391; C:Elg1 RFC-like complex; IPI:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0031389; C:Rad17 RFC-like complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IDA:SGD.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0006272; P:leading strand elongation; IDA:SGD.
 GO:0006298; P:mismatch repair; TAS:SGD.
 GO:0007062; P:sister chromatid cohesion; IPI:SGD. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR008921; DNA_pol3_clamp-load_cplx_C.
 IPR027417; P-loop_NTPase.
 IPR013748; Rep_factorC_C_dom. 

Pfam

 PF00004; AAA
 PF08542; Rep_fac_C 

SMART

 SM00382; AAA 

PROSITE

  

PRINTS